Postdoctoral Research Associate We are seeking to appoint a Postdoctoral Research Associate to join our team.

3+ year #postdoc #job with Keith Matthews, in Edinburgh, studying #trypanosome virulence and transmission. Using a combination of bioinformatics and wet lab techniques.

Share wide and apply here: elxw.fa.em3.oraclecloud.com/hcmUI/Candid...

A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená

The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6

A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená

The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6

A Trypanosoma brucei cell visualized by confocal microscopy after ultrastructure expansion. The cell was immunolabelled for the BILBO1 protein (yellow) and stained with fluorescent NHS-ester (grey). The flagellar pocket is highlighted in pink. Credit: Marie Zelená

The flagellar pocket collar (FPC) is a cytoskeletal structure essential for nutrient uptake & immune evasion in #Trypanosome. @mbonhivers.bsky.social &co use U-ExM to provide novel insights into FPC biogenesis, and reveal 2 unknown cytoskeletal structures @plosbiology.org 🧪 plos.io/4n3bWi6

Intraflagellar transport selectivity occurs within the proximal portion of the #trypanosome flagellum, say Aline Araujo Alves, Philippe Bastin and colleagues @bastinlab.bsky.social @pasteur.fr: rupress.org/jcb/article/...

#Microbiology #Cilia #Trafficking #StructuralBiology #Flagella

Drs @singh-parul.bsky.social @trypsleon.bsky.social‬ Maureen Cabahug @fariatrypslab.bsky.social & @jessmbryant.bsky.social review #Plasmodium & #Trypanosoma #genome organization.

#Trypanosome #Transcription #Chromatin #Epigenetics #Nuclear #Compartment

www.cell.com/trends/paras...

Inhibitor fluorination pattern tunes chemically induced protein dimerization Chemically induced dimerization of proteins is a powerful approach to regulate biomolecular functions through small molecule ligands acting as 'molecular glues'. Here, we demonstrate that simple, thie...

A one day double whammy for our very own Eric in #trypanosome research! 🧪🥳

Backbone assignments of the essential oxidoreductase from T. cruzi (Chagas disease): tinyurl.com/ywshhxjt 🧲🦟

and his master piece on the role of fluorine in chemically induced dimerization:
www.biorxiv.org/content/10.1...

Mass Spectrometry Reveals Novel Features of Tubulin Polyglutamylation in the Flagellum of Trypanosoma brucei Tubulin polyglutamylation is a key feature of eukaryotic cilia and flagella that is essential for their function. The diversity of enzymes catalyzing polyglutamylation with different specificities ins...

Finally out! Mass spec analysis of #trypanosome flagellar tubulins reveals some surprises including a new #glutamylation site on beta-tubulin! Many thanks to the teams of Julia Chamot-Rooke and Mariette Matondo!
pubs.acs.org/doi/10.1021/...

Ella presenting on trypanosome cell biology

Thanks to Rochdale Sixth Form College, Settle College and Cardinal Newman College for taking part in our #Biosciences Masterclass. Research students Hannah Pyle and Ella Southern delivered a taster lecture on #trypanosomiasis (a #NeglectedTropicalDisease caused by #trypanosome parasites) (1/2)

Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin:
-a new glutamylation site
-far less exhaustive detyrosination

Preprint here:
www.biorxiv.org/content/10.1...

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Expansion microscopy identifies novel asymmetric pore proteins. The images show proExM of cell lines co-expressing epitope-tagged versions of NUP76 (top; magenta), NUP149 (middle; magenta), or NUP140 (bottom; magenta) in combination with NUP110 (green). Images were deconvolved (20 iterations for NUP149 and NUP76; 60 iterations for NUP140) and single planes of the nuclei are shown.

How is transport directionality achieved through the #trypanosome nuclear pore complex? The current model is symmetrical, but this study reveals that the trypanosome #NuclearPore has an asymmetrical architecture, with conserved hubs that direct mRNA transport 🧪 @plosbiology.org plos.io/3Q36QV2

Welcome to Blue Sky @dewarlab.bsky.social - give Caroline a follow for all things #trypanosome and #mitochondria . Her shiny new group is just being established @blslancasteruni.bsky.social - exciting research to come 🎉

Structural basis of Spliced Leader RNA recognition by the Trypanosoma brucei cap-binding complex - Nature Communications Bernhard et al. reveal the architecture of the tetrameric Trypanosoma brucei nuclear cap-binding complex. Using cryo-electron microscopy and biochemical assays, the researchers characterize its bindin...

#CapBindingComplex #CBC #Trypanosome #RNA #RNAsky

www.nature.com/articles/s41...

Exciting PhD project in the lab of Sue Vaughan at #Oxford Brookes University to study #trypanosome basal bodies and flagellum formation
www.jobs.ac.uk/job/DKY713/n...

Congratulations to Jean Marc @tsagmoj.bsky.social who won an Early Career Entomologist Award from the @royentsoc.bsky.social for his PhD work on tsetse fly midgut composition and its possible impact on #trypanosome transmission! 💪
Read more here:
www.royensoc.co.uk/res-journal-...

1 day left to apply: Available PhD position @DiMeN_DTP @The_MRC
How does #SUMOylation shape nuclear organisation? How does that impact #trypanosome immune evasion.
If you find this an exciting topic, join us! @BiologyatYork @YBRI_UoY & @UoDLifeSciences
For more information, contact me!

This is some very beautiful work from Manu's group

#Trypanosome #JBC #structuralbiology

Here we are! To celebrate our arrival, here is a #trypanosome expanded ~13 times & stained for tubulin, individual cortical #microtubules can be recognised, what was only achievable by EM before. Images: Christine Girard-Blanc & Thierry Blisnick. Many thanks to @centriolelab.bsky.social for training

🎓A 2 years funded post-doc position is available in the Trypanosome Cell Biology Unit at @institutpasteur Paris🔬 Project TrypaDiff - Glycerol, a new key player in the #trypanosome parasite cycle

🗓️Starting Mar. 1, 25 - Application before Nov. 30, 24

research.pasteur.fr/en/team/group-…

Chagas disease

Here’s a series of Lancet articles (some #OpenAccess and others currently accessible without subscription) on #Chagas disease, “a silent zoonotic disease caused by Trypanosoma cruzi, a #parasite with a remarkable life cycle” #Tcruzi #trypanosome #NTD 🧪
www.thelancet.com/series/chaga...

Glycerol, a possible new player in the biology of trypanosomes Citation: Bringaud F, Plazolles N, Pineda E, Asencio C, Villafraz O, Millerioux Y, et al. (2021) Glycerol, a possible new player in the biology of trypanosomes. PLoS Pathog 17(12): e1010035. https://doi.org/10.1371/journal.ppat.1010035

In this new Pearl Article, @BringaudF and his team highlight recent data on glycerol #metabolism and their implications for understanding #trypanosome biology : "Glycerol, a possible new player in the #biology of trypanosomes". In @PLOSPathogens

dx.plos.org/10.1371/journa…

First paper for @SougalFreitas, @ParaFrap #PhD student, in Infect Genet Evol @sciencedirect. The team studied the link between diversity & abundance of the bacterial content of the fly's gut and the #trypanosome infection status. @InterTryp @ird_fr #HAT

sciencedirect.com/science/articl…

Positional dynamics and glycosomal recruitment of developmental regulators during trypanosome differentiation Glycosomes are peroxisome-related organelles that compartmentalise the glycolytic enzymes in kinetoplastid parasites. These organelles are developmentally regulated in their number and composition, allowing metabolic adaptation to the parasite’s needs in the blood of mammalian hosts or within their arthropod vector. A protein phosphatase cascade regulates differentiation between parasite developmental forms, comprising a tyrosine phosphatase, TbPTP1, that dephosphorylates and inhibits a serine threonine phosphatase TbPIP39 that promotes differentiation. When TbPTP1 is inactivated, TbPIP39 is activated and during differentiation becomes located in glycosomes. Here we have tracked TbPIP39 recruitment to glycosomes during differentiation from bloodstream stumpy forms to procyclic forms. Detailed microscopy and live cell imaging during the synchronous transition between life cycle stages revealed that in stumpy forms, TbPIP39 is located at a periflagellar pocket site closely associated with TbVAP, that defines the flagellar pocket endoplasmic reticulum. TbPTP1 is also located at the same site in stumpy forms, as is REG9.1, a regulator of stumpy-enriched mRNAs. This site provides a molecular node for the interaction between TbPTP1 and TbPIP39. Within 30 minutes of the initiation of differentiation TbPIP39 relocates to glycosomes whereas TbPTP1 disperses to the cytosol. Overall, the study identifies a ‘stumpy regulatory nexus’ (STuRN) that co-ordinates the molecular components of life cycle signalling and glycosomal development during transmission of Trypanosoma brucei . Importance African trypanosomes are parasites of sub-Saharan Africa responsible for both human and animal disease. The parasites are transmitted by tsetse flies and completion of their life cycle involves progression through several development steps. The initiation of differentiation between blood and tsetse forms is signalled by a phosphatase cascade, ultimately trafficked into peroxisome-related organelles called glycosomes that are unique to this group of organisms. Glycosomes undergo substantial remodelling of their composition and function during the differentiation step but how this is regulated is not understood. Here we identify a cytological site where the signalling molecules controlling differentiation converge before the dispersal of one of them into glycosomes. This coincides with a specialised ER site that may contribute to glycosome developmental biogenesis or regeneration. In combination, the study provides the first insight into the spatial co-ordination of signalling pathway components in trypanosomes as they undergo cell-type differentiation.

New publication in Derrick Robinson’s Team : Positional dynamics and glycosomal recruitment of developmental regulators during #trypanosome differentiation @biorxivpreprint #science #research #parasitology

biorxiv.org/content/10.110…