@simoncleary.bsky.social, Longhui Qiu, Mark Looney et al. developed a new #intravital technique for imaging pulmonary lymphatics. rupress.org/jem/article/...

📘 In our #Lymphatics collection: rupress.org/jem/collecti...

🔬Our AX MP multiphoton confocal system is ideal for intravital microscopy and deep tissue imaging.

👀Ready to unlock new imaging possibilities?

👉Discover what AX MP can do: https://bit.ly/4sqwODR

#NikonMicroscopy #confocal #multiphoton #intravital #microscopy #mp

Our Jove video is finally out! Watch us try our best to look natural while reading the script 🤭

#vascular #imaging #bonemarrow #leukemia #intravital #twophoton #passarolab @institutcochin.bsky.social @imag-ic.bsky.social

app.jove.com/v/67332/intr...

We are very proud of our latest collaboration with the @doctordianap.bsky.social team and the company @ymetry.bsky.social for the development of titanium-printed implants aimed at improving the reproducibility of #intravital imaging through #multiphoton #microscopy.
app.jove.com/t/67332/intr...

The updated version of our latest manuscript on #endothelial-driven #extravasation is available. We use single cell #intravital imaging in #zebrafish to monitor #mechanochemical Ca2+ signaling driving #actin remodeling required for active #endothelium CTC extrusion.
www.biorxiv.org/content/10.1...

LKB1 acts as a critical brake for the glucagon-mediated fasting response As important as the fasting response is for survival, an inability to shut it down once nutrients become available can lead to exacerbated disease and severe wasting. The liver is central to transitions between feeding and fasting states, with glucagon being a key initiator of the fasting response in the liver. However, the precise regulatory mechanisms behind glucagon-induced fasting are unclear. One potential mediator of these transitions is Liver Kinase B1 (LKB1) given its role in nutrient sensing. Here, we show LKB1 knockout mice have a severe wasting and prolonged fasting phenotype despite increased food intake. By applying RNA sequencing and intravital microscopy we show that loss of LKB1 leads to a dramatic reprogramming of the hepatic lobule through robust upregulation of periportal genes and functions. This is mediated through the opposing effect LKB1 has on glucagon signaling and gene expression. Conclusion: our findings show that LKB1 acts as a brake to the glucagon-mediated fasting response resulting in “periportalization” of the hepatic lobule and whole-body metabolic inefficiency. These findings reveal a new mechanism by which hepatic metabolic compartmentalization is regulated by nutrient-sensing. ### Competing Interest Statement The authors have declared no competing interest. * LKB1 : Liver Kinase B1 NAFLD : Non-Alcoholic Fatty Liver Disease GLP-1 : Glucagon-like peptide 1 PP : Periportal PC : Pericentral AMPK : AMP-activated Protein Kinase ARK : AMPK-related KO : Knock out WT : Wild Type PCK1 : phosphoenolpyruvate carboxykinase 1 GAPDH : Glyceraldehyde 3-phosphate dehydrogenase AAV8 : Adeno-Associated Virus 8 TBS : Tris-buffered saline TBST : Tris-buffered saline triton PBS : Phosphate-buffered saline FBS : Fetal Bovine serum PFA : Paraformaldehyde I.p. : intraperitoneally BW : Body weight FGF21 : Fibroblast growth factor 21 ACC1 : Acetyl-CoA carboxylase NaCl : Sodium Chloride 2-NBDG : 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose PCR : Polymerase chain reaction qPCR : Quantitative polymerase chain reaction DEG : Differential expression of genes GSEA : Gene set enrichment analysis NIDAP : NIH Data Analysis Portal IPA : Ingenuity pathway analysis FC : Fold change FDR : False discovery rate

I'm excited to share our first paper investigating the role of LKB1 in regulating the fasting response and liver zonation led by Sueheley Acevedo, @MeganStefkovich @SunWooSophieKa1 @RoryCunningham_ #liver #Intravital

biorxiv.org/content/10.110…

LKB1 acts as a critical brake for the glucagon-mediated fasting response As important as the fasting response is for survival, an inability to shut it down once nutrients become available can lead to exacerbated disease and severe wasting. The liver is central to transitions between feeding and fasting states, with glucagon being a key initiator of the fasting response in the liver. However, the precise regulatory mechanisms behind glucagon-induced fasting are unclear. One potential mediator of these transitions is Liver Kinase B1 (LKB1) given its role in nutrient sensing. Here, we show LKB1 knockout mice have a severe wasting and prolonged fasting phenotype despite increased food intake. By applying RNA sequencing and intravital microscopy we show that loss of LKB1 leads to a dramatic reprogramming of the hepatic lobule through robust upregulation of periportal genes and functions. This is mediated through the opposing effect LKB1 has on glucagon signaling and gene expression. Conclusion: our findings show that LKB1 acts as a brake to the glucagon-mediated fasting response resulting in “periportalization” of the hepatic lobule and whole-body metabolic inefficiency. These findings reveal a new mechanism by which hepatic metabolic compartmentalization is regulated by nutrient-sensing. ### Competing Interest Statement The authors have declared no competing interest. * LKB1 : Liver Kinase B1 NAFLD : Non-Alcoholic Fatty Liver Disease GLP-1 : Glucagon-like peptide 1 PP : Periportal PC : Pericentral AMPK : AMP-activated Protein Kinase ARK : AMPK-related KO : Knock out WT : Wild Type PCK1 : phosphoenolpyruvate carboxykinase 1 GAPDH : Glyceraldehyde 3-phosphate dehydrogenase AAV8 : Adeno-Associated Virus 8 TBS : Tris-buffered saline TBST : Tris-buffered saline triton PBS : Phosphate-buffered saline FBS : Fetal Bovine serum PFA : Paraformaldehyde I.p. : intraperitoneally BW : Body weight FGF21 : Fibroblast growth factor 21 ACC1 : Acetyl-CoA carboxylase NaCl : Sodium Chloride 2-NBDG : 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose PCR : Polymerase chain reaction qPCR : Quantitative polymerase chain reaction DEG : Differential expression of genes GSEA : Gene set enrichment analysis NIDAP : NIH Data Analysis Portal IPA : Ingenuity pathway analysis FC : Fold change FDR : False discovery rate

I'm excited to share our first paper investigating the role of LKB1 in regulating the fasting response and liver zonation led by Sueheley Acevedo, @MeganStefkovich @SunWooSophieKa1 @RoryCunningham_ #liver #Intravital

biorxiv.org/content/10.110…

Excited to share @MeganStefkovich and @SunWooSophieKa1 protocol!
Learn about the tools they developed for #intravital #imaging of the liver and glucose mobilization.

x.com/Curr_Protocols…

Excited to share @MeganStefkovich and @SunWooSophieKa1 protocol!
Learn about the tools they developed for #intravital #imaging of the liver and glucose mobilization.

x.com/Curr_Protocols…

Dynamic polyhedral actomyosin lattices remodel micron-scale curved membranes during exocytosis in live mice Nature Cell Biology - Using intravital imaging, Ebrahim et al. show that actin and non-muscle myosin II assemble into polyhedral lattices around the vesicle membrane to mediate exocytic secretion...

Dr. Seham Ebrahim discovered #actomyosin lattices associated with secretory granules. Amazing #intravital microscopy at high resolution, check it out here:

nature.com/articles/s4155…

Dynamic polyhedral actomyosin lattices remodel micron-scale curved membranes during exocytosis in live mice Nature Cell Biology - Using intravital imaging, Ebrahim et al. show that actin and non-muscle myosin II assemble into polyhedral lattices around the vesicle membrane to mediate exocytic secretion...

Dr. Seham Ebrahim discovered #actomyosin lattices associated with secretory granules. Amazing #intravital microscopy at high resolution, check it out here:

nature.com/articles/s4155…

Why #mitochondria move and how it affects their function? Are mitochondria dynamic in intact tissues, in vivo? How would that change under physiological and pathological conditions?
My lab uses #intravital #microscopy to address these questions - postdoc position available!

Why #mitochondria move and how it affects their function? Are mitochondria dynamic in intact tissues, in vivo? How would that change under physiological and pathological conditions?
My lab uses #intravital #microscopy to address these questions - postdoc position available!