PuckerLab header in Easter flavour. Details: https://www.izmb.uni-bonn.de/en/pbb

🌱🐣 Happy Easter from our Plant Biotechnology & Bioinformatics Group! 🧬

Celebrating renewal, curiosity, and the hidden “Easter eggs” in biological data 🧪💻

🌿 Happy Easter! 🌼
www.izmb.uni-bonn.de/en/pbb

#Genomics #Bioinformatics #PlantSciences
@bpucker.bsky.social @nancy-choudhary.bsky.social

Postdoctoral Research Scientist (Plant Engineering Biology)

Applications are open for a #postdoc researcher to join Dr Sarah Guiziou at Earlham Institute working on plant #engineeringbiology for sustainable agriculture.

This project aims to develop integrase circuits to engineer root architecture.

#PlantSciences #EngBio

@sarah-guiziou.bsky.social

Time projection of microtubules in maize epidermal cells

#PlantSciences

Check out Nancy's latest work about the long history of anthocyanin research and the strong growth of this research topic 👇 #PlantSciences #Evolution

Various images showing plant and root phenotypes of geralt mutant Arabidopsis plants

A helping hand....🆘

Agnieszka K Banaś et al. have identified a plant-specific photolyase/blue-light receptor 2 family protein termed GERALT, which is critical for the early development and function of #chloroplasts in #Arabidopsis 🌱

🔗 doi.org/10.1093/pcp/...

#PlantSciences

And so it begins... #Monogram26

For the second year, the ECR workshop organised by
@nikkiwalter.bsky.social was very well attended...

Lovely to host so many Monogram researchers at @niab-uk.bsky.social for a day.

#plantsciences #crops

Thank you @sev1panier.bsky.social for the shoutout.
I highly recommend reading their fantastic work on one of the most challenging areas in #plantsciences: quantitative disease resistance (QDR).An honour to cover this work as the @theplantcell.bsky.social AFE @aspbofficial.bsky.social

Lara

Our new group member Lara focuses on cloning arGSTs from plants! 🧬✨ Thanks to Najnin for excellent support in the lab.

www.izmb.uni-bonn.de/en/pbb/team

#PlantSciences #Puckerlab #UniBonn #PlantBiotechnology #MolecularBiology #Anthocyanins #Evolution

Discover top and trending #PlantSciences research in BioOne Complete featuring articles from Cryptogamie, Mycologie @mnhn.fr, Lindbergia, and Novon @mobotgarden.bsky.social.

Free to read through March 31: bio-one.co/3NzW2jc

#PlantScience #PlantBiology #botany #moss #fungi #flower #plants #scisky

Tuning of the RBR1-E2F/DP transcriptional module by the F-box protein FBL17 FBL17 regulates the RBR1–E2F/DP transcriptional module to prevent aberrant activation of the DNA damage response in Arabidopsis.

Tuning of the RBR1-E2F/DP transcriptional module by the F-box protein FBL17 | Science Advances www.science.org/doi/10.1126/...
#plantsciences

Plant genome sequencing workflow. Details: https://doi.org/10.1186/s12864-026-12623-z

Need a genome sequence of your favourite plant? We laid out instructions for a complete workflow based on ONT sequencing:

"Cookbook for plant genome sequences"
doi.org/10.1186/s128...

#Genomics #LongReads #PlantSciences #Bioinformatics
@puckerlab.bsky.social @unibonn.bsky.social

Regulation of endosymbioses by CEP and CLE signaling peptides in Medicago truncatula On the left (green background), regulation of nitrogen-fixing nodulation by signaling peptides in Medicago truncatula. On the right (orange background), regulation of mycorrhization by signaling peptides in M. truncatula.

Have a look to the new review from the F. Frugier’s SILEG team @IPS2ParisSaclay, published in Trends in Plant Sciences, about signaling peptides acting at the crossroad of plant root endosymbioses. #PlantSciences ips2.u-psud.fr/en/articles/...

Bruno Studer argues that strict NGT laws favor big corporations by creating high entry barriers. To keep Swiss breeding local and innovative, we need science-based regulation, not more hurdles. 🌾🚫 Thank you, "Schweizer Bauer", for the comprehensive interview! 👇

#PlantSciences #NGT

Innovations in spatial imaging could unlock higher wheat yields Scientists at the Earlham Institute and the John Innes Centre are pioneering powerful single-cell visualisation techniques that could unlock higher yields of global wheat.

Published in @theplantcell.bsky.social scientists at Earlham Institute and @johninnescentre.bsky.social use #spatial transcriptomics to reveal gene expression patterns in developing #wheat inflorescences.

Read more 👉 buff.ly/a5nWTXx

#PlantSciences #Genomics

Comprehensive benchmarking of tools for nanopore-based detection of DNA methylation Long read sequencing technologies such as Oxford Nanopore (ONT) offer direct, simultaneous detection of DNA base modifications. The recent migration of ONT to the upgraded R10 chemistry has spurred the development of diverse methylation detection models. However, their performance and accuracy remain unclear. Here, leveraging diverse bacterial, plant, and mammalian datasets, we systematically evaluate the current landscape of tools and models for studying DNA methylation using nanopore sequencing. Our results demonstrate that the older models remain the best choice for studying CpG methylation. We note substantial improvement of newer tools in identifying 5-methylcytosine in non-CG contexts, 6-methyladenine, and 4-methylcytosine. We highlight the sensitivity of various tools to confounding methylation nearby. We also assess the computational performance of various tools, and effects of sequencing depth, methylation abundance, read quality, and basecalling mode. Our reusable pipelines and fully open access datasets provide a framework of resources to empower future benchmarking efforts. Our work thus details the strengths and limitations of the state-of-the-art methylation models and outlines practical guidelines for researchers using nanopore sequencing to study DNA modifications. ### Competing Interest Statement DTS has participated as a speaker in events organized by Oxford Nanopore Technologies. LZ is currently an employee of Oxford Nanopore Technologies. However, his association with this work predates his current employment. Other authors declare no competing interests. Rockefeller Foundation, https://ror.org/03sfkwk85, 2021 HTH 018 Department of Biotechnology, https://ror.org/03tjsyq23, BT/PR40264/BTIS/137/44/2022

Comprehensive benchmarking of tools for nanopore-based detection of DNA methylation
doi.org/10.1101/2024...

Cookbook for Plant Genome Sequences
www.doi.org/10.20944/pre...

#Genomics #Bioinformatics #PlantSciences

Chromosome counting from root tips of a Kalanchoe species

Kalanchoe leaf section stained with DAPI (blue) and an RNA probe (red) for a constitutive, highly expressed gene.

Section of a young, emerging foliar embryo from Kalanchoe.

No context microscopy pictures. #PlantSciences

Someone got Cas9-Turbo to work. It's like basically Y1H but with proteomics. It however requires transgenic plant expressing Cas9-Turbo and gRNAs, which is still cumbersome for many plants that are not Arabidopsis. #PlantSciences

I urge you to watch and learn from Deep Down and Dirty: the Science of Soil.

Don’t mess with the stuff!

#EnvironmentalScience #PlantSciences #Fungi #Soil

www.youtube.com/watch?v=gYXo...

Just 2 days left for teams of students on #PlantSciences degree programmes in Britain & Ireland to register for #BUC2026!
First round of Botanical University Challenge is on 11 Feb online so save that date.
@bsbibotany.bsky.social is proud to be a @bucbotany.bsky.social supporter again this year!

Selection of ecological functions of anthocyanins: Aposematism, Pathogens, Camouflage, Batesian mimicry, Anti-camouflage. Details: https://doi.org/10.1186/s12870-025-08006-3

What is the major role of anthocyanins—painting flowers, acting as sunscreens, responding to stress, or defending against pathogens?

Our latest paper dives into the diversity and ecological functions of anthocyanins 🌈🌿

doi.org/10.1186/s128...

#PlantSciences #Anthocyanins @bpucker.bsky.social

Tree showing relationships of LAR genes. Details: https://doi.org/10.1186/s12864-025-12429-5

Excited to share that Maria's work on the evolution of the LAR gene has been published in BMC Genomics:

Evolutionary dynamics of the proanthocyanidin biosynthesis gene LAR
doi.org/10.1186/s128...

#Evolution #FlavonoidBiosynthesis #PlantSciences
@bpucker.bsky.social @maferrecinos.bsky.social

Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis - Nature Communications Targeted DNA methylation editing is critical for establishing the causal relationship between DNA methylation and its function as well as for epigenetic crop breeding. Here, the authors develop a CRIS...

Two papers on targeted DNA methylation editing for Arabidopsis came out within a week from each other.

1. www.nature.com/articles/s41...
2. academic.oup.com/pcp/advance-...

#PlantSciences

#PlantSciences #Crops #PlantHormones #miRNA @camplantsci.bsky.social

I’m very happy to share that next year I’m launching the Meristem Hydraulics Group. We will be at the @umeaplantsciencecentre.se (Sweden) & the University of Helsinki (Finland) @helsinki.fi

Here is a brief description of our research interests:
www.upsc.se/juan_alonso_...

#PlantSciences

What are good enzymatic methyl-seq kits nowadays (for plants)? #PlantSciences #Epigenetics

Sounds very cool! #PlantSciences

The B-class auxin response factor MpARF2 is essential for meristem organization in free-living plant gametophytes Land plants (embryophytes) are multicellular eukaryotes with a remarkable capacity to grow continuously during their life span. They achieve this by m…

Out today... From Bowman lab, MpARF2 is the first essential transcription factor to be described for meristem maintenance in Marchantia. Glad to have contributed to another paper with Eduardo. #plantsciences #evodevo www.sciencedirect.com/science/arti...

Fantastic opportunity to study one of the less-understood while most-exciting symbiotic system in plants! #plantsciences #postdoc

Geoffrey and his “Barley Audible” poster at the PGR showcase #barley #sound #acoustics #engineering #plants #plantsciences @engineering-soton.bsky.social

Prof Dr Henrik Hartmann, Head of the JKI Institute for Forest Protection. Photo: Johannes Kaufmann/JKI

👏 Prof Dr Henrik Hartmann (Head of JKI Institute for Forest Protection; joint professorship at the University of Göttingen; former group leader @bgc-jena.mpg.de) has once again been listed in 2025 as a #HighlyCited #Researcher! Congrats! 👏
clarivate.com/highly-cited...
#PlantSciences @clarivate.com