@gl-hooper.bsky.social

10 months ago

Frontiers | Broad-spectrum coronavirus inhibitors discovered by modeling viral fusion dynamics Development of oral, broad-spectrum therapeutics targeting SARS-CoV-2, its variants, and related coronaviruses could curb the spread of COVID-19 and avert fu...

New study uses computational modelling + pseudotyped virus assays to identify broad-spectrum antivirals that block coronavirus cell entry. A step forward in rapid antiviral development.
www.frontiersin.org/journals/mol...
#Virology #PseudotypedVirus #DrugDiscovery #COVID19 #Pseudotyping

@gl-hooper.bsky.social

10 months ago

🧬This study used a luciferase-expressing influenza virus pseudotype to track infection in mice via bioluminescent imaging, following immunisation with a quadrivalent influenza vaccine.
www.mdpi.com/1999-4915/17...
#Influenza #PseudotypedVirus #VaccineResearch #Virology #Pseudotyping

@gl-hooper.bsky.social

11 months ago

Novel Trispecific Neutralizing Antibodies With Enhanced Potency and Breadth Against Pan‐Sarbecoviruses Multispecific antibodies harness the advantages of two or more mAbs. In this study, we designed novel trispecific antibodies, Tri-1 and Tri-2, derived from our previously identified mAbs, PW5-5, PW5-...

🔬Novel tri-specific antibodies demonstrate potent neutralisation of SARS-CoV-2 variants and related sarbecovirus pseudotyped viruses.🧬

onlinelibrary.wiley.com/doi/10.1002/...
#Virology #Pseudotyping #PseudotypedVirus #Abs #Antivirals #COVID19 #SARSCoV2 #VirusNeutralisation

@gl-hooper.bsky.social

11 months ago

HPV16 neutralizing monoclonal antibodies show evidence for common developmental pathways and public epitopes Antibodies to human papillomavirus (HPV) primarily recognize surface exposed residues on five loops of the major capsid protein (L1) that vary significantly among HPV types. We determined which loops ...

This recent preprint used HPV16 viral pseudotypes to explore the diversity and molecular characteristics of human monoclonal antibodies (mAbs) generated following HPV vaccination.
#Virology #Pseudotyping #PseudotypedVirus #HPV #mAbs #Vaccination
www.biorxiv.org/content/10.1...

@gl-hooper.bsky.social

11 months ago

Assessing anti-rabies vaccine response in humans: A rapid and high-throughput adaptable, pseudovirus-based neutralization assay as an alternative to rapid fluorescence focus inhibition test (RFFIT) Author summary Rabies causes approximately 59000 deaths every year world-wide. It is also among one of the neglected tropical diseases aimed to achieve ‘Zero by 30’ as per the UN Sustainable Developme...

🧬Rabies Pseudotype Neutralisation Assay: A Safer, Faster Way to Monitor Vaccine Responses

journals.plos.org/plosntds/art...
#RABV #Rabies #Pseudotyping #PseudotypedVirus #Virology #HighThroughPut #NeutralisationAssay #PublicHealth #VaccineResearch

@gl-hooper.bsky.social

11 months ago

Frontiers | FDA-approved drug repurposing screen identifies inhibitors of SARS-CoV-2 pseudovirus entry

🧪 Drug repurposing using pseudotyped virus
A recent study used a SARS-CoV-2 pseudotyped virus system to screen drugs for potential inhibitors of viral entry. frontiersin.org/journals/pha...
#PseudotypedVirus #SARSCoV2 #Virology #AntiviralResearch #COVID19 #VirusEntry #Pseudotyping

@gl-hooper.bsky.social

1 year ago

Neutralization and spike stability of JN.1-derived LB.1, KP.2.3, KP.3, and KP.3.1.1 subvariants | mBio The emergence of novel severe acute respiratory syndrome coronavirus 2 variants continues to pose challenges for global public health, particularly in the context of immune evasion and viral stability...

🧬 DelS31 Mutation: A Key Player in COVID-19 Evolution 🧬
This study investigates the impact of the DelS31 mutation in JN.1-derived COVID-19 variants.
journals.asm.org/doi/10.1128/...
#COVID19 #SARSCoV2 #Pseudotyping #PseudotypedVirus #Vaccine

@gl-hooper.bsky.social

1 year ago

🚨 New study on broad-spectrum HIV-1 inhibitors!
Tested via pseudotyped virus & cell fusion assays, these NPIs offer promising potential.
🔗www.sciencedirect.com/science/article/pii/S266...
#Virology #Pseudotyping #HIV #Antivirals #DrugDiscovery

Virion X

@virionx.bsky.social

1 year ago

🚨 We Want to Hear from You! 🚨Please take a moment to complete our quick 5-question survey. It will only take a minute, but your input will help shape the future of our work.
👉https://virion-dde96pr8.scoreapp.com
#Virology #Research #VirionX #PseudotypedVirus #Pseudotyping #biotech

@gl-hooper.bsky.social

1 year ago

This study explored Exifone, a clinical polyphenolic compound, as a potential treatment for COVID-19. It binds to the SARS-CoV-2 3CL protease and disrupts ACE2/S-RBD interaction. lnkd.in/e7sMyMcB
#Pseudotyping #Antiviral #COVID19 #Virology #SARSCoV2 #PseudotypedVirus

Virion X

@virionx.bsky.social

1 year ago

🚀 Exciting news! We’re launching Virion X, a biotech dedicated to making critical virology research more accessible. We’re building a waitlist so you’ll be the first to know when we go live. lnkd.in/emSCjXcY
#VirionX #Virology #Pseudotyping #Biotech #startup #pseudotypedvirus

@gl-hooper.bsky.social

1 year ago

Function and structure of broadly neutralizing antibodies against SARS-CoV-2 Omicron variants isolated from prototype strain infected convalescents - Journal of Translational Medicine Background The ongoing emergence of evolving SARS-CoV-2 variants poses great threaten to the efficacy of authorized monoclonal antibody-based passive immunization or treatments. Developing potent broa...

This study identifies potent mAbs with strong neutralising activity against SARS-CoV-2. 2YYQH9 & LQLD6HL reduced lung pathology in XBB.1.16-infected hamsters, showing promise for future therapies. #pseudotyping #COVID19 #mAbs #virology🔗 translational-medicine.biomedcentral.com/articles/10....

@gl-hooper.bsky.social

1 year ago

This preprint uses pseudotyped virus deep mutational scanning to map HIV escape mutations against two broadly neutralising antibodies, 10-1074 & 3BNC117, revealing strain-dependent differences.
#HIV #virology #antibodies #pseudotyping #pseudotypedvirus
www.biorxiv.org/content/10.1...

@gl-hooper.bsky.social

1 year ago

Population shift in antibody immunity following the emergence of a SARS-CoV-2 variant of concern - Scientific Reports Scientific Reports - Population shift in antibody immunity following the emergence of a SARS-CoV-2 variant of concern

This paper looks at population immunity to SARS-CoV-2 in south Africa following the emergence of the Beta VOC, using spike binding and pseudotyped virus neutralisation assays. #virology #pseudotyping #COVID19 #Immunology #Antibodies www.nature.com/articles/s41...

@gl-hooper.bsky.social

1 year ago

Bridge protein-mediated viral targeting of cells expressing endogenous μ-opioid G protein-coupled receptors in the mouse and monkey brain Targeting specific cell types is essential for understanding their functional roles in the brain. Although genetic approaches enable cell-type-specifi…

Novel Bridge Protein Strategy for Cell-Specific Delivery of Pseudotyped Viruses.
#virology #pseudotyping #pseudotypedvirus #tropsim #proteinengineering
www.sciencedirect.com/science/arti...

@gl-hooper.bsky.social

1 year ago

Antiviral Mechanisms and Preclinical Evaluation of Amantadine Analogs that Continue to Inhibit Influenza A Viruses with M2S31N-Based Drug Resistance To better manage seasonal and pandemic influenza infections, new drugs are needed with enhanced activity against amantadine- and rimantadine-resistant…

Amantadine analogues inhibit drug-resistant influenza A: blocking pseudotyped virus entry & disrupting M2-M1 interactions. Promising for future antivirals for drug-resistant IAV. www.sciencedirect.com/science/arti... #Influenza #Antivirals #DrugResistance #Virology #pseudotyping

@gl-hooper.bsky.social

1 year ago

Recombinant protein HR212 targeting heptad repeat 2 domain in spike protein S2 subunit elicits broad‐spectrum neutralizing antibodies against SARS‐CoV‐2 and its variants 1. HR212 assembles to be a trimer with three HR2s exposed. 2. The nAbs induced by HR212 showed inhibition of HR1 binding with HR212 and the nAbs induced by HR212 would block the S2 conformational rea....

Targeting SARS-CoV-2 spike HR2: Researchers designed HR212, a recombinant protein that inhibits viral infection across 28 pseudotyped variants. This highlights its potential as a broad-spectrum antiviral. #COVID19 #virology #pseudotyping #antiviralresearch onlinelibrary.wiley.com/doi/10.1002/...

@gl-hooper.bsky.social

1 year ago

Development and application of a high-titer VSV-based HCoV-NL63 pseudovirus system via C-terminal 14 amino acid truncation of spike To provide efficient tools for the development of novel antiviral drugs and vaccines of HCoV-NL63, it is urgently necessary to establish a safe, widel…

Developing a high-titre VSV-based pseudotyped virus for HCoV-NL63, researchers enhanced infection efficiency by truncating the spike protein, which they use for antiviral screening. www.sciencedirect.com/science/arti... #Virology #Pseudotyping #DrugDiscovery

@gl-hooper.bsky.social

1 year ago

Replication-incompetent VSV-based vaccine elicits protective responses against SARS-CoV-2 and influenza virus Bivalent replication-incompetent VSV vaccine provides protection against both SARS-CoV-2 and influenza.

A new bivalent VSV-based vaccine targets SARS-CoV-2 & influenza A, inducing strong immune responses in mice. It protects against both viruses & cross-protects against flu strains, offering promise for a broad-spectrum respiratory vaccine. #Vaccines #pseudotyping www.science.org/doi/10.1126/...

@gl-hooper.bsky.social

1 year ago

Nanoscopic acoustic vibrational dynamics of a single virus captured by ultrafast spectroscopy | PNAS The natural vibrational frequencies of biological particles such as viruses and bacteria encode critical information about their mechanical and bio...

Nano-acoustics could enable viral fingerprinting via ultrafast spectroscopy. Vibrations at 19–21 GHz reveal morphology/protein interactions, while 2–10 GHz modes track disassembly. This label-free method could advance diagnostics and research. #virology #pseudotyping www.pnas.org/doi/10.1073/...

@gl-hooper.bsky.social

1 year ago

Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner - Veterinary Research Porcine deltacoronavirus (PDCoV), also known as HKU15, is a swine enteropathogenic virus that is believed to have originated in birds. PDCoV belongs to the genus Deltacoronavirus (DCoV), the members o...

This recent study used pseudotyped virus assays to show that DCoVs enter DF-1 cells via clathrin-mediated endocytosis, relying on clathrin, dynamin-2, cathepsins, and low pH, while independent of caveolae/macropinocytosis. #pseudotyping #virology
veterinaryresearch.biomedcentral.com/articles/10....

@gl-hooper.bsky.social

1 year ago

Protocol for evaluating humoral immune responses in mice following SARS-CoV-2 vaccination Binding and neutralizing antibodies are critical indicators of protection against viral pathogens and are essential for assessing the immunogenicity a…

This paper outlines a protocol to assess humoral immune responses in mice post-SARS-CoV-2 vaccination. It features ELISA for spike-specific binding antibodies and a pseudotyped virus neutralization assay for neutralizing titers. #pseudotyping #COVID19 #vaccine
www.sciencedirect.com/science/arti...

@gl-hooper.bsky.social

1 year ago

Development of a latency model for HIV-1 subtype C and the impact of long terminal repeat element genetic variation on latency reversal Sub-Saharan Africa accounts for almost 70 % of people living with HIV (PLWH) worldwide, with the greatest numbers centred in South Africa where 98 % o…

Maikoo et al. have developed a HIV-1C latency model. Revealing that the HIV-1C consensus LTR exhibited a lower reactivation potential compared to its HIV-1 subtype B counterpart.
#Virology #pseudotyping #HIV
www.sciencedirect.com/science/arti...

@gl-hooper.bsky.social

1 year ago

Diketopiperazine/piperidine alkaloid as a potential broad-spectrum coronaviral entry inhibitor identified by supercomputer-based virtual screening from a large natural product-based library The COVID-19 pandemic has underscored the urgent need for antiviral agents capable of targeting a broad range of coronaviruses, including emerging var…

This study identified a natural diketopiperazine/piperidine alkaloid that inhibits pseudotyped and live SARS-CoV-2, SARS-CoV-2 XBB, SARS-CoV, MERS-CoV, and HCoV-HKU1 by targeting spike protein RBD-ACE2 interactions. #COVID19 #pseudotyping www.sciencedirect.com/science/arti...

@gl-hooper.bsky.social

1 year ago

Host cell lectins ASGR1 and DC-SIGN jointly with TMEM106B confer ACE2 independence and imdevimab resistance to SARS-CoV-2 pseudovirus with spike mutation E484D | Journal of Virology The interaction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein with the ACE2 receptor determines the viral cell tropism and is the key target of the neutralizing antibod...

A recent study revealed SARS-CoV-2 variants with the E484D spike mutation can bypass ACE2, using host lectins ASGR1 and DC-SIGN for cell entry. This pathway resists imdevimab treatment and could impact therapeutic strategies.
journals.asm.org/doi/10.1128/...
#pseudotyping #COVID19

@gl-hooper.bsky.social

1 year ago

Simultaneous Blockade of CD209 and CD209L by Monoclonal Antibody Does Not Provide Sufficient Protection Against Multiple Viral Infections In Vivo Many virus species, including Ebola virus, Marburg virus, SARS-CoV-2, DENV and ZIKV, exploit CD209 and CD209L as alternative or attachment receptors for viral infection. The 7-H7-B1 mAb, which blocks...

This study used pseudotyped and live viruses to assess the chimeric mAb 7-H7-B1, which blocks CD209 and CD209L. 7-H7-B1 effectively inhibited infections from SARS-CoV, SARS-CoV-2, Ebola, Marburg, ZIKV, and DENV in vitro. #pseudotyping #virology
onlinelibrary.wiley.com/doi/10.1111/...

@gl-hooper.bsky.social

1 year ago

A Global Collaborative Comparison of SARS-CoV-2 Antigenicity Across 15 Laboratories Setting up a global SARS-CoV-2 surveillance system requires an understanding of how virus isolation and propagation practices, use of animal or human sera, and different neutralisation assay platforms...

A recent study analysed neutralisation assays across 15 labs using the WHO SARS-CoV-2 antibody standard. Three live neutralisation assays and a pseudotyped assay showed excellent consistency, including between live and pseudotyped virus assays.
#pseudotyping #virology www.mdpi.com/1999-4915/16...

@gl-hooper.bsky.social

1 year ago

Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations

This preprint employed pseudotyped virus deep mutational scanning to examine how mutations in the rabies glycoprotein affect cell entry and neutralisation by eight monoclonal antibodies. #pseudotyping #rabv www.biorxiv.org/content/10.1...

@gl-hooper.bsky.social

1 year ago

Re-Engineered Pseudoviruses for Precise and Robust 3D Mapping of Viral Infection Engineered vesicular stomatitis virus (VSV) pseudotyping offers an essential method for exploring virus–cell interactions, particularly for viruses that require high biosafety levels. Although this ap...

ClickVSV, a fluorescent pseudotyped vesicular stomatitis virus, enables the visualisation and tracking of cell binding and entry in living cells, unlike conventional pseudotyped viruses. pubs.acs.org/doi/10.1021/...
#pseudotyping #virology #fluorescence

@gl-hooper.bsky.social

1 year ago

Guardians at the Gate: Optimization of Small Molecule Entry Inhibitors of Ebola and Marburg Viruses Ebola and Marburg (EBOV and MARV) filoviral infections lead to fatal hemorrhagic fevers and have caused over 30 outbreaks in the last 50 years. Currently, there are no FDA-approved small molecule ther...

This recent paper utilised Marburg and Ebola virus pseudotypes to develop small molecule entry inhibitors at CL-2, before validating and optimising hits with live virus at CL-4, perfectly demonstrating the usefulness of pseudotyped viruses
pubs.acs.org/doi/10.1021/...
#pseudotyping #virology