HHMI Investigators Michael Rosen (L) and Eric Gouaux (R) and collaborators Rosana Collepardo-Guevar (L) and Magdalena Schneider (R) examine a cryo-electron microscope at our Janelia Research Campus, a key tool in developing AI-powered imaging inside cells.

#HHMIInvestigators Eric Gouaux & Michael Rosen are using AI to turn a powerful cellular imaging tool into a molecular detective, revealing hidden molecular details in cryo-ET images to better understand how cellular structures work, & how they may malfunction: bit.ly/47Q5u9v.

How do you get a charged drug into a cell when charged molecules can't cross the membrane? You don't — you let the cell do the charging. Check our new paper "Charged molecular glue discovery enabled by targeted degron display" in Nature Chemical Biology.

❕Publication: www.nature.com/articles/s41...

Excited to share our work on the structure and function of cytoplasmic lattices within mouse embryos. A collaborative effort with @niakanlab.bsky.social and work led by @kashishsingh.bsky.social and @inaharasimov.bsky.social . It is now out on BioRxiv: www.biorxiv.org/content/10.6...

In situ cryo-ET of mammalian embryos reveals cytoplasmic lattices contain ubiquitin-charged E2-E3 ligase assemblies www.biorxiv.org/content/10.64898/2026.03.22.713481v1 #cryoEM

The E3 ubiquitin ligase mechanism specifying target-directed microRNA degradation (TDMD) is now published! 🎉🍾 We, @bartellab.bsky.social and Schulman lab, describe how 2-RNA factors control protein degradation by recruiting an E3 ligase. @mpibiochem.bsky.social
www.nature.com/articles/s41...

We're excited to announce the beta-release of #RELION-5.1! 🥳 This release has important updates on our #amyloid processing pipeline, as described in this preprint: doi.org/10.64898/202... There are also some tweaks for #teamtomo.

#OpenSoftwareAcceleratesScience

Cryo-EM image processing of amyloid filaments in RELION-5.1 www.biorxiv.org/content/10.64898/2026.03.17.712386v1 #cryoEM

ChimeraX screenshot showing CubeNTube plugin.

The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....

One first decision after diagnosis was that I would continue: continue to work, continue to have fun, continue to laugh, continue to hope. Luckily I was always a person with a quite digital life. Using eye tracking you can still do a lot. Especially as a software developer.

Hi #cryoEM - The Thermo Fisher Scientific software team has opened their 2026 feedback survey for #EPU, Tomography 5, and more.

⏱️ ≤20 min, skip what’s not relevant. All experience levels welcome!

Help us prioritize future developments and improvements:
👉 forms.office.com/pages/respon...

Super exciting to see this preprint come out! It’s been a wonderful opportunity to be a part of this story and to apply Surface Morphometrics tooling to a fascinating system. Congratulations to all authors @wilflinglab.bsky.social
#teamtomo

New year, new preprint! 🎊

We are excited to share our recent work on #E3 ligase regulation in #metabolism!

www.biorxiv.org/content/10.6...

#ubiquitin #targetedproteindegradation #chemicalbiology

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We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉

When RNA Degradation 🤝 meets 🤝 Protein Degradation! tinyurl.com/E3TDMD In a collaboration of @bartellab.bsky.social and Schulman lab, we show that, in target-directed microRNA degradation (TDMD), 2-RNA-factors recruit an E3 ligase and induce the degradation of not only a protein but also RNA (1/5).

Final version is out! Our large-scale cryo-ET dataset 🔬 of Chlamydomonas reinhardtii 🦠 is now published in @cp-molcell.bsky.social

Huge collaborative effort! So glad to see the community already using it to develop new resources & tools.

Check it out here: shorturl.at/z4i4c
#CryoEM #CryoET

We are thrilled to share our new pre-print on the molecular anatomy of nuclear proteasome in human sperm cells! #In-cellcryo-ET + #SPA + #LM! @piotrkolata.bsky.social @a-dsantos.bsky.social, @tomdendooven.bsky.social!! doi.org/10.64898/202...

Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁‍♀️💎✨VIP section💅🪩🥂

Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.

s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com

The mechanistic basis of cargo selection during Golgi maturation Resident proteins of the Golgi recycle in vesicles and the protein GOLPH3 enables the COPI vesicle coat to accomplish this.

The mechanistic basis of cargo selection during Golgi maturation. From Rebecca Taylor, @katciazynska.bsky.social, @jggkaufman.bsky.social & @grigorytagiltsev.bsky.social at @mpibiochem.bsky.social with David Owen and Sean Munro's group @cellbiol-mrclmb.bsky.social | www.science.org/doi/10.1126/...

CryoSift – An accessible and automated CNN-driven tool for cryo-EM 2D class selection

Check out our new preprint from the @landerlab.bsky.social, Stagg-Lab and Cianfrocco-Lab: CryoSift - An accessible and automated CNN-driven tool for cryo-EM 2D class selection www.biorxiv.org/content/10.1...
Happy testing: cryosift.org #cryoEM #CNN

#TeamTomo ❄️🔬 meets #MassSpec Imaging 🧬🔬. Super cool, and great potential for EM grids with mixes of different cell types. For example -- environmental samples! @embltrec.bsky.social 🌍 🧪

𝗖𝘂𝗿𝗶𝗼𝘂𝘀 𝗵𝗼𝘄 𝘄𝗲 𝘂𝘀𝗲 𝗵𝗶𝗴𝗵-𝘁𝗲𝗰𝗵 𝘁𝗼 𝗮𝗱𝘃𝗮𝗻𝗰𝗲 𝗼𝘂𝗿 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵?
Then join us at our Open Day and get exciting sneak peaks behind the scenes of one of Europe’s largest life science campuses in Martinsried.

Find out more (in German): mpi.lineupr.com/tdot/

Very excited to announce that my first, first author paper, is now on BioRxiv! In this paper we unambiguously show #COPII coated vesicles in unperturbed human cells for the very first time!!

Make my day and check it out!
www.biorxiv.org/content/10.1...

#Cryo-ET #TeamTomo #Cryo-CLEM ❄️🔬❄️🔬❄️🔬❄️🔬

Vitrification is THE make-or-break for cryoET in tissues. Each sample we figure out opens so many new possibilities for understanding biology. Great screening effort, rewarded with beautiful tomograms. Congrats!

Awesome plant root #CryoET (native Casparian strip) and a detailed methods paper (silver needle cryo-lift out) all in one-- 13 figures! Jean is a machine! Congrats @chgenoud.bsky.social @nikogeldner.bsky.social and the DCI team. 🧪🧶🧬🌾🔬

Schulman lab is ready for the GRK2243 Symposium: Understanding ubiquitination: from molecular mechanisms to disease in würzburg

#wUeBI2025 @grk2243.bsky.social
@jakobfarnung.bsky.social @samuelmaiwald.bsky.social @hannahbkmpr.bsky.social

Cryogenic light microscopy of vitrified samples with Angstrom precision www.biorxiv.org/content/10.1101/2025.05.27.656160v1 #cryoEM

TRIP12 structures reveal HECT E3 formation of K29 linkages and branched ubiquitin chains - Nature Structural & Molecular Biology Using biochemistry, chemical biology, and cryo-EM, Maiwald et al. elucidate how TRIP12 forms K29 linkages and K29/K48-linked branched ubiquitin chains, revealing a mechanism for polyubiquitylation sha...

Excited to share our latest study on how K29/K48-branched #ubiquitin chains are forged by the #E3 ligase TRIP12, and how this suggests a consensus mechanism for chain formation by HECT E3s!

@natsmb.nature.com

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www.nature.com/articles/s41...

John Briggs has been elected to the Fellowship of the Royal Society, the UK’s national academy of sciences. Congratulations to him and the other outstanding researchers from across the world!

❕https://royalsociety.org/news/2025/05/new-fellows/

@briggsgroup.bsky.social ‪@royalsociety.org

Congratulations @mmedina300kv.bsky.social 🫶🎉🍾🎊

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

We are excited to present our latest work published in @natcomms.nature.com

www.nature.com/articles/s41...