A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNAS The Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation www.pnas.org/doi/10.1073/...

The ribosome-associated N-terminal acetyltransferase B coordinates global proteostasis and autophagy in plants by creating Ac/N-degrons - Nature Communications Protein N-terminal acetylation profoundly influences protein fate. Here, the authors establish NatB as a central regulator of the interplay between ubiquitin–proteasome system and autophagy, and highl...

Resistance to prolonged darkness in plants steered by N-terminal acetylation | New study by Gong et al. Markus Wirtz lab @giglionelab.bsky.social Tanja Bange lab #NatB #autophagy #N-degron #proteostasis www.nature.com/articles/s41...

NAC promotes co-translational protein folding at the ribosomal tunnel exit Santos et al. reveal NAC’s co-translational interactome, showing compartment-specific nascent chain interactions in human cells. NAC engages emerging chains at the ribosomal tunnel exit via a ribosome-oriented hydrophobic pocket, promoting early domain folding without stabilizing intermediate structures. These findings establish NAC as a co-translational chaperone integrating folding with protein biogenesis.

Online Now: NAC promotes co-translational protein folding at the ribosomal tunnel exit Online now:

Congratulations Jake @mediocre-jake.bsky.social, Bekky Feltham and team on their phenomenal effort in putting together the E3-ome, published in Cell www.cell.com/cell/fulltex....

An extremely valuable resource for ubiquitin researchers.

NAA40 and NAC cooperate in co-translational histone acetylation in humans Nature Communications - N-terminal histone acetylation by human NAA40 alters epigenetic signaling and affects gene regulation. Here, the authors combine biochemistry and cryo-EM to unravel the...

How are histones acetylated as they emerge from the ribosome? We show that NAA40 cooperates with the NAC complex to enable co-translational acetylation of H2A/H4, revealing NAC as a coordinator of nascent protein modification. Read more about our collaboration with @kirmizislab.bsky.social #cryoEM

Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry Highly efficient hyperthermal acidic proteases combined with hybrid-fragmentation schemes provide five times more unique peptide reads than trypsin or chymotrypsin, greatly boosting confidence in de n...

Deep coverage and extended sequence reads obtained with a single archaeal protease expedite de novo protein sequencing by mass spectrometry: Cell Systems www.cell.com/cell-systems.... How great would it be to sequence proteins without a gene-template! We might be approaching this soon.

The Golgi Network, Volume II This book explores the interaction of Golgi with different organelles such as ER, the vesicular system, and protein complexes that execute these tasks.

A wonderful collection of contemporary topics and perspectives on secretion and Golgi biology edited by Akihiko Nakano and Jaakko Saraste.
The Golgi Network, Volume II @SN and link.springer.com/book/10.1007...

Co-translational control of protein stability and quality in plants Abstract. Proteostasis relies on the coordinated control of protein synthesis, folding, modification and degradation, and an increasingly clear picture is

My review “Co-translational control of protein stability and quality in plants” is now online at @jxbotany.bsky.social, in which I describe how co-translational processing and ribosome-associated quality control together establish protein stability and fate early in synthesis. tinyurl.com/5dhhz9h6

Indeed! Feel free to suggest here or to ISPT. Also, many speakers will be selected from Abstracts so this route is open.

8 days left to register | 28 invited speakers | Keynotes from F. Ulrich Hartl, Roland Beckmann and Michael Rapé | #chaperones #degradation #ubiquitin #cryoEM #acetylation #lipidation #ribosomes #proteins
proteintermini.org/meeting/

Actin N-terminal maturation can be blocked by a butynamide stereoprobe which acts as an ACTMAP inhibitor. Preprint by Xiong et al. Benjamin Cravatt and Bruno Melillo. @ispt-proteinterm.bsky.social
www.biorxiv.org/content/10.6...

Join us in Palermo!
FEBS Workshop Protein Termini 2026: the power of protein termini across bacteria, plants, and animals—from ribosome biology to proteostasis and applications. Deadline: 3 March 2026
proteintermini.org/meeting
#ProteinTermini #Proteostasis #FEBS #EMBO @iubmb.bsky.social

Join us at the @crick.ac.uk for the 2026 meeting of the UK proteostasis community!
We especially encourage students and postdocs to attend and share their work. All talks (except the keynotes) will be selected from abstracts.

Surprisingly: "Conditional stability of HY5 through the ATE N-degron pathway regulates environmental responses in Arabidopsis thaliana".
The shining bounds of N-degron pathway influence expands! @charlene-kunaka.bsky.social www.biorxiv.org/content/10.6...

Protein event of the year - sign up today! See you at beautiful Palazzo dei Normanni in Palermo for the FEBS 2026 Protein Termini Workshop.
#ProteinTermini #Proteostasis #ProteinModifications #StructuralBiology #PalazzoDeiNormanni #Palermo2026
proteintermini.org/meeting/

Remember to register for this year's Protein Termini FEBS Advanced course. Keynote by Roland Beckmann @beckmannlab.bsky.social "It takes more than two to tango: structural basis and coordination of co-translational N-terminal nascent chain modification" www.conferencecentral.org/webpage/view...

Protein degradation in Turnip - an adapted Arg/N-degron pathway. Preprint from Brian Mooney et al. Emmanuelle Graciet lab. www.biorxiv.org/content/10.6...

Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors Protein biogenesis requires the ribosome to collaborate with a diverse set of cotranslational factors that shape the fate of nascent chains. These int…

Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors. New review out in @cp-molcell.bsky.social from Elke Deuerling's lab. www.sciencedirect.com/science/arti...

Complexity at the ribosomal polypeptide tunnel exit. The major N-terminal acetyltransferase NatA may form different complexes on the ribosome to facilitate protein maturation. By Marius Klein & Klemens Wild in Irmgard Sinning's lab BZH & Nina McTiernan in my lab. www.nature.com/articles/s41...

This is how co-translational N-terminal myristoylation occurs on the ribosome. NMT1 acts together with NAC, but after the release of MetAP. Cryo-EM study by Timo Denk et al. @beckmannlab.bsky.social @giglionelab.bsky.social www.nature.com/articles/s41...

🎉 As we celebrate #TIBS50, we're highlighting #CitedClassics and revisiting the top cited article from each of the last 50 years!

Check out this one from 1976! Read it here 👉 lnkd.in/egdN-AXt

1. Apply for one of the positions below (lab or theory) by Jan 18th
2. Learn interdisc skills and discover cool new things about how mitochondria move and socialise
3. Explore some of Norway's beautiful nature (both the below, Rundemanen and Gullfjellet, <10km from work)

Intricate regulation of Src kinases via N-terminal modifications and specific degradation pathways. A novel degradation pathway involving the CUL4A-DDB1-DCAF10 E3 ligase found by Kremer et al. Tanja Bange lab @andrea-musacchio.bsky.social @ispt-proteinterm.bsky.social www.nature.com/articles/s41...

Mechanism of cotranslational modification of histones H2A and H4 by MetAP1 and NatD NAC enables cotranslational N-terminal processing of histones H2A and H4 by recruiting MetAP1 and NatD at the ribosomal tunnel.

Last X-Mas, the ribosome gave you methionine,
but the very next day, MetAP took it away.
This year, to save histones from tears,
NatD gives you an acetyl group. ⭐️

Explore our latest paper with the Deuerling lab @uni-konstanz.de and Shu-ou Chan lab @caltech.edu!

www.science.org/doi/10.1126/...

HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA Huntingtin-interacting protein K (HYPK) promotes the activity of N-terminal acetyltransferase A (NatA), which cotranslationally acetylates 40% of the eukaryotic proteome. Kinetic and in-cell measurements revealed that HYPK acts as a ribosome exchange factor for NatA, enabling its access to and acetylation of the translatome.

Online Now: HYPK promotes N-terminal protein acetylation through rapid ribosome exchange of NatA Online now:

📢 Our December issue is now online! Access the full issue 👉 www.cell.com/trends/bioch...

🎨 The cover brings warm, summertime vibes ☀️ and highlights a Feature Review on #AromaticAminoAcid biosynthesis in 🌱 from Jorge El-Azaz and @hiroshi-maeda.bsky.social. Cover art credit to Tae Park.

(1/n)

We’ve made some new tools to manipulate N-recognins. Check them out in our preprint.
www.biorxiv.org/content/10.1...

Just registered for FEBS advanced workshop Protein termini 2026 in Palermo #proteintermini2026 (?) Looking forward to a great meeting. @ispt-proteinterm.bsky.social
www.conferencecentral.org/webpage/view...

Our study led by the extraordinary multitasking Daai Zhang shows here (tinyurl.com/3uhbkh54) that an additional O2 sensing mechanism based on histone methylation helps roots to prepare for potentially lethal hypoxic stress (such as in waterlogging)