Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullination Laposchan et al. report a motif-based strategy to generate monoclonal antibodies for global detection of protein citrullination. The antibodies show strong sensitivity and selectivity over homocitrull...

Not the most obvious project for a mass spec lab...but here we are, making antibodies.😀
Our new paper reports two motif-based mAbs that broadly detect citrullination, distinguish it from homocitrullination, and work in ELISA, WB, and IF. www.cell.com/cell-reports...
#citrullination #antibodies #PTM

This review highlights protein #citrullination as a key #PostTranslationalModification influencing #ChromatinRemodeling & gene transcription, outlining its physiological roles & recent advances in understanding its interplay with other modifications.

Read: doi.org/10.1016/j.ge...

High-throughput chemical proteomics workflow for profiling protein citrullination dynamics - Nature Communications Protein citrullination is linked to autoimmunity and inflammation but is challenging to detect globally. Here, the authors report a high-throughput chemical proteomics workflow and demonstrate its uti...

Our proteomics workflow for profiling citrullination dynamics is out 🎉
www.nature.com/articles/s41...
Idea from a journal club discussion + Funding + Determined PhD student + many “this didn’t work…” moments =
A robust, accessible citrullinome enrichment workflow
#proteomics #citrullination #PTM

Citrullination in health and disease: From physiological function to gene regulation

This review summarizes the physiological functions of protein #Citrullination and outlines the recent progress in understanding its role in #genetranscription regulation, particularly in relation to other #PostTranslationalModifications. #medsky

#OpenAccess: www.sciencedirect.com/science/arti...

We of course couldn’t let that slip and developed a method:
✅ compatible with tandem MS
✅ strong enrichment efficiency
✅ uses commercial reagents, works in tubes + 96-well plates
We hope it becomes your new favorite for hunting this tiny PTM 🔥
#citrullination #proteomics #ptms

Our Motif-Specific mAbs...
✔ Motif-specific (and sensitive)
✔ Minimal homocitrullination cross-reactivity
✔ Works in real samples
Also? Some commercial Abs didn’t stand a chance. 😉
To try? We’re making ours openly available to support the citrullination community!
#Citrullination #Antibodies #PTMs

Many congratulations Chien-Yun and team! Great to see this out. Prosit-Cit is an important addition to the the community’s toolkit for studying #citrullination

Deep Learning Enhances Precision of Citrullination Identification in Human and Plant Tissue Proteomes Citrullination is a critical yet understudied post-translational modification (PTM) implicated in various biological processes. Exploring its role in health and disease requires a comprehensive unders...

Kicking off the new year strong! Prosit-Cit is officially online!🍻 Check it out: www.mcponline.org/article/S153...

#DeepLearning #Citrullination #Bioinformatics

Our work on histone #citrullination, which is found as much outside the nucleus/cell as it does within, led us to wonder how often #histones and #chromatin are found to be extranuclear/extracellular.

The answer is often, across different tissues and across the tree of life.

Do take a read, especially if you're interested in #peptides, #covalentdrugs, #geneticcodereprogramming #argininedeiminases or #citrullination

Thanks to coauthors Ewen Calder and Simone Kunzelmann and to everyone else that contributed!

@imperialchemistry.bsky.social @crick.ac.uk