Stabilization of IQGAP1 by the mTORC2 component PRR5 mediates mitogenic LINC01133-to-ERK signaling in triple-negative breast cancer A lincRNA promotes TNBC growth by activating the kinases AKT and ERK through an mTORC2 component.

A team of researchers has discovered how an RNA promotes the growth of triple-negative breast cancer (#TNBC) by activating two distinct molecular pathways and identified a new combination treatment that suppresses TNBC in culture. #SciSignal https://scim.ag/4rotdW4

Phosphorylation of RYR1 at Ser2902 decreases Ca2+ leak in skeletal muscle and susceptibility to malignant hyperthermia and heat stroke Reducing Ca2+ leak through mutant RYR1 prevents pathological heat production in skeletal muscle.

New #scisignal paper: Phosphorylation of RYR1 at Ser2902 decreases Ca2+ leak in skeletal muscle and susceptibility to malignant hyperthermia and heat stroke. #Myoblue tinyurl.com/bddss3j6

#TuberousSclerosisComplex #PulmonaryArterialHypertension

#MatrixStiffness 25 kPa-induced loss of TSC2 in PA #SmoothMuscleCell➡️
⏫mTOR & #YAP/TAZ➡️
⏫PASMC proliferation & ECM deposit (➡️fibroblast proliferation)

SM22a+ SMC het KO🐭of Tsc2➡️PAH at 9-wk

#SciSignal 2022
www.science.org/doi/10.1126/...

Note that #EndothelialCell miR-15a/16-1 KO has similar cerebrovascular benefits - Angiogenesis & BBB

#CircRes 2020
www.ahajournals.org/doi/10.1161/...

#SciSignal 2020
www.science.org/doi/10.1126/...

Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry Community-suggested best practices for the analysis of oxylipins are presented.

Technical recommendations for analyzing oxylipins by liquid chromatography–mass spectrometry #SciSignal www.science.org/doi/10.1126/...

#Microtubule #SmoothMuscleCell Contractility

MT support #SarcoplasmicReticulum - #PlasmaMembrance contact

RyR2 channel clusters on tubulin filaments

Nocodazole
⏫Ca2+ spark spread
⏬RyR2-BKα co-localization
⏫myogenic tone

@vesselman.bsky.social #SciSignal 2017
www.science.org/doi/10.1126/...

#SIRT6 #AlzheimersDisease

#AmyloidPrecursorProtein as a deacetylase target of SIRT6➡️ubiquitin-proteasomal degradation

MDL-800, (Sirt6 activator)
⏬Cortical + Hippocampal APP Aβ40 Aβ42
⏫Cognitive behaviors Nest-building/NOR/Water maze
in APP/PS1🐭

#SciSignal 2024
www.science.org/doi/10.1126/...

Class II PI3K/PI3K-C2β as a NEGATIVE regulator of mTORC1 activity (likely via regulating its Lysosomal localization) & #EndothelialCell Size during👁️Retinal #Angiogenesis
Cool story😎

Are those enlarged EC senescent, flow-insensitive, or leaky?😆

#SciSignal 2023
www.science.org/doi/10.1126/...

#GouldSyndrome

Col4a1 G1344D/+🐭
Misfolded collagen protein #ERStress->
Age-related⏬RyR- & IP3R-mediated ER/SR Ca2+ signaling->
⏬#MyogenicTone in Cerebral artery

A fascinating paralog to recent ACTA2 R149C mutation-> #ERStress-> #Atherosclerosis story
#SciSignal 2023
www.science.org/doi/10.1126/...