Discover how to use the ibidi µ-Slide Spheroid Perfusion in our ibidi Handling Video🎥

🔍 If you are working with 3D models and flow-based assays, this is a clear step-by-step introduction to get started.

Watch here: zurl.co/pTlMN

#howtovideo #microscopy #spheroids

New to 3D Cell Culture?

Learn about 3D cell cultures, why and how they are used, and how to choose assays for 3D models on our website, which includes a free 3D Culture Guide at: https://shorturl.at/vllVN

#3DCellCulture #CellCulture #spheroids

#Unlock the power of 3D cell culture with the ibidi Pump System🔓

The ibidi Pump System provides stable long-term #perfusion under physiological conditions for #organoids, #spheroids, and organ-on-a-chip models 🧠🫀🫁🦴

📖Learn more: zurl.co/7DpB3

#pumpsystem #innovation #technology

From Zero to Monopoly: The Asymmetric Warfare of Organic Network Dominance. How Platform Economics Creates Winner-Take-All Markets Without Traditional Competition. From Zero to Monopoly: The Asymmetric Warfare of Organic Network Dominance How Platform Economics Creates Winner-Take-All Markets Without...

better-experience.blogspot.com/2026/01/from...

#LEUKOENCEPHALOPATHY #WITH #NEUROAXONAL #SPHEROIDS
multi-search-tag-explorer.aepiot.com/advanced-sea...
#ZOE #SLATER
aepiot.com/advanced-sea...
#MINISTRY OF #PESHMERGA #AFFAIRS
multi-search-tag-explorer.aepiot.com/advanced-sea...
aepiot.com

🎄December #Scaffold: Image of the Month#❄️

#Patterned growth supports tissue-like #spheroids and #organoids that better mimics real tissue. The scaffold was 3D-printed via vat polymerization.

🥼🔬Scientific #artwork by Mina Aleemardani from @bristoluni.bsky.social , UK.

#sciart #bioart

Demonstrating an #Acoustofluidic pick-and-place operation system capable of spatially assembling #Spheroids into desired patterns in both 2D and 3D spaces, advancing #TissueEngineering, #TumorModeling, & #RegenerativeMedicine.

#IJEM #OpenAccess: doi.org/10.1088/2631...

In this video, you'll get an introduction to 3D cell cultures, including #spheroids, #tumorspheres, and #organoids.

Watch our full webinar to learn about our machine-learning based application for simple, automated organoid counts!

buff.ly/TUc9kLd

Back with a🆕ibidi Tips & Tricks🔧

👩‍🔬Marie, who is a PhD student, shares a clean, low-stress setup for #connecting the µ-Slide Spheroid Perfusion to↔️the ibidi Pump System while keeping #spheroids and #organoids stable under flow🛡️🫧

🔗https://zurl.co/mCTVj

#tipsandtricks #3Dcellculture #flowassay

An illustration of approximately 13 spheroids, small floating aggregates of cells, that can be used at scale in the lab to test medicines in development. Watercolour and black biro on paper.

#spheroids can vary considerably in size and shape, yet to be used in drug discovery, they must be as consistent as possible to produce meaningful results.

I'm turning this #illustration into Holiday Decorations - watch this space! 🐡 🎨 #biosky 🎄❄️

Aquaporin 5 expression regulates MDA-MB-231 spheroid multicellular invasion | American Journal of Physiology-Cell Physiology | American Physiological Society Aquaporins are water transport proteins that regulate pro-metastatic behaviors in cancer, including cell invasion, proliferation, and epithelial-to-mesenchymal transition. Aquaporin 5, an isoform virtually absent from healthy tissues, is overexpressed in numerous cancer types. Studies have connected Aquaporin 5 to the migratory and invasive properties of single cells in two-dimensional assays; however, the role of Aquaporin 5 in inducing invasive phenotypes in models that recapitulate the tumor microenvironment remains unknown. To address this gap, MDA-MB-231 cell lines were created with Aquaporin 5 overexpression and knockdown to identify the resulting single and collective cell motility in three-dimensional models. Upon validating the developed cell lines, Aquaporin 5 expression regulated cell motility and invasion in varied biaxial microenvironments. Additionally, Aquaporin 5 was found to play a unique role in regulating MDA-MB-231 spheroid development, influencing the formation, size, circularity, and adhesion, distinct from its function in two-dimensional models. Finally, increased Aquaporin 5 expression intensified the invasive capacity of spheroid multicellular protrusions by polarizing to their invasive front. In summary, this work expands upon the knowledge that Aquaporin 5 enhances cell motility, while elucidating the previously unreported adhesive and multicellular invasive effects of Aquaporin 5 in a three-dimensional model.

Using human #spheroids, researchers at @univofmaryland.bsky.social, @medschool.umaryland.edu, & @gtresearch.bsky.social uncovered new roles for Aquaporin 5 in cancer invasion, offering deeper insight into tumor behavior & potential therapeutic targets.

doi.org/10.1152/ajpc...

#oncsky 🧪

UPR-induced intracellular C5aR1 promotes adaptation to the hypoxic tumour microenvironment - Cell Death & Disease Cell Death & Disease - UPR-induced intracellular C5aR1 promotes adaptation to the hypoxic tumour microenvironment

What if we’ve been aiming at the wrong surface?

Under <0.1% O₂, UPR drives intracellular C5aR1 that supports tumour cell survival — favouring cell-permeable inhibitors. #GelCount used to track 3D spheroid growth and to image/analyze clonogenic plates.

#Hypoxia #Spheroids #ClonogenicAssay

Taking a shot here, a colleague is seeking a #postdoc position in/near #Germany. She’s eager to expand into #organoids , & has experience in #Spheroids, #BiologicalImaging, #CellBiology #HighContentMicroscopy
Any leads, connections, or suggestions would be hugely appreciated!
#Microscopy

2️⃣ Pool the spheroid suspension and embed the #spheroids into approximately 100% Matrigel® domes at a density of 70 - 100 free-floating spheroids per dome.

Let the Matrigel® solidify at 37°C for 10 minutes.

Instructions:

1️⃣ Generate mid/hindgut #spheroids following the STEMdiff™ Intestinal Organoid Kit Technical Manual (harvested on Day 7/8).

#MSLASpheroidStamp: #OpenSource 3D #cell #spheroids for everyone:

- #3Dprinted molds/stamps
- time-lapse #confocal #microscopy of spheroids
- #cytotoxicity screens

Preprint: doi.org/10.1101/2024...
GitHub: github.com/arteys/MSLAS...
#DIYbio #lab #instruments #MSLA #3Dprinting #bioprinting #imaging

Second day of #EACR2025 coming to an end!
Another great day of talks about, well, really many different things but mostly #organoids #spheroids #ai #drugtreatments #precisionmedicine
Tomorrow join our talk Label-free spheroids and organoids analysis for assays development
11:15 - 11:30 !

The MSLASpheroidStamp project includes 3D printed stamps that can be used to make indentations in agarose for #cell_culture #spheroids directly, and silicone molds (similar to MicroTissue).

3/n

My first post on just appeared paper #rheology #cancer #spheroids in #collagen #hydrogels - pubs.acs.org/toc/abseba/0/0 - conducted with a #rheometer, not with #AFM this time, :-).
@felixrico.bsky.social

A fibroblastic network growing out of a #PDAC #tumor #spheroids shared by our scientist Tom from his recently published vascularized, PDAC model. Would like to learn how to set up such a model? Join our next advanced course on “#Cancer and immune cell integration” in May. #AACR

LinkedIn This link will take you to a page that’s not on LinkedIn

🧬 A new application of 𝗦𝗽𝗵𝗲𝗿𝗼𝗧𝗿𝗶𝗯𝗲!

In colorectal cancer (CRC), Mismatch Repair Deficiency (MMRd) is common and leads to poor responses to anti-PD-1 therapy, with up to 50% of MMRd CRC patients failing treatment.
🧪 The SpheroTribe kit was employed to grow compact spheroids .

#spheroids

🔬 Get to know Thomas DAUBON and explore his SpheroTribe adventure!
🔗 Dive into his full story here: lnkd.in/eFcXZThQ

#science #innovation #discover #spheroids #research

#Cancer #EMT #Oncology #CellBiology #Spheroids #Biomechanics #Metastasis #CancerResearch #Cell

2️⃣ Pool the spheroid suspension and embed the #spheroids into approximately 100% Matrigel® domes at a density of 70 - 100 free-floating spheroids per dome.

Let the Matrigel® solidify at 37°C for 10 minutes.

Instructions:

1️⃣ Generate mid/hindgut #spheroids following the STEMdiff™ Intestinal Organoid Kit Technical Manual (harvested on Day 7/8).

The PDAC-on-Chip - A New Dimension in Pancreatic Cancer Research A new biochip replicates key aspects of the PDAC microenvironment, providing a powerful tool for drug development and reduction of animal testing.

[𝐍𝐞𝐰 𝐛𝐥𝐨𝐠] The PDAC-on-Chip – A New Dimension in Pancreatic Cancer Research. Read a summary of the most important findings of our recent study with the team of Prof. Dr. Nicole Teusch at the University of Düsseldorf here: zurl.co/7pdot
#drugdevelopment #cancer #spheroids #preclinic

So pleased that this powerhouse of tech and training that can do so much to replace #AnimalResearch now exists in lil Ole Blighty!
#organoids #spheroids

Stem cell & 3D cell culture novel technologies for modern developmental biology, regenerative medicine & cell therapy

🔹#StemCell Maintenance & Differentiation
🔹#Organoids, #Spheroids & #3CellCulture
🔹#Antibodies for Stem Cell & Development
🔗 tinyurl.com/2p9t574z

#biotech #biopharma #bioscience

🩺 Veratridine & Colorectal #Cancer (CRC)🌸

A new study highlights #Veratridine, a plant-derived alkaloid, as a potential treatment for CRC. It inhibits a hyperactive signaling pathway, inhibiting angiogenesis, and slowing tumor growth. #SpheroTribe was used to study its effects on #spheroids.

High-throughput bioprinting of spheroids for scalable tissue fabrication - Nature Communications HITS-Bio, a high-throughput bioprinting platform, rapidly assembles spheroids to mimic native tissue architecture. Its intraoperative application in bone shows near-complete calvarial defect repair in...

High-throughput #bioprinting of #spheroids for #scalable #tissue #fabrication,🧪

www.nature.com/articles/s41...

Congrats Ruslan Dmitriev on the early career award from The Histochemical Society! it was great to see you present on your work on #spheroids and #oxygen imaging and #FLIM!