Oh wow.

Some fraction of AMPAR heteromers incorporate CNIH1-4 in HEK cells. That was always my fear about switching to *another* cell line- we would not know the mixture.

CNIH continues to be interesting…

#iglurs

Wow so cool congrats Hiro and team! #iglurs

Very happy to take some time to travel to Marseille and Montpellier to present our latest work on #iglurs

I’ll be at INMED tomorrow, AMU on Tuesday and Montpellier on Friday.

If you’re in the area, and fancy meeting up, let me know.

Hmmm, yes. Interesting results.

Looks like the reviews are not published though. That's quite unusual these days.

I'm not sure I agree that Claudin24 is "powerful" in the sense described in the abstract!

#iglurs

Slow AMPA receptors in hippocampal principal cells - PubMed Glutamate receptor ion channels, including α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, mediate fast excitatory neurotransmission in the CNS. Previous work suggested that AMPA receptors produce a synaptic current with a millisecond duration. However, we find that about two- …

Seth Grant mentions that many synapses do not have NMDA receptors- a key fact that many ignore.

Shameless self promotion: some synapses (in many brain regions) do have terrifically powerful slow AMPA receptors.

See our 2021 paper:

pubmed.ncbi.nlm.nih.gov/34348150/

#iglurs #SynapticDiversity

Efficient and rapid isolation of native AMPA receptor complexes for cryo‐EM Isolating native ion channels for structural characterization is routinely achieved by extraction from membrane fractions of tissue with prolonged mild detergent treatment. AMPA receptors (AMPARs), g...

New fast protocol for GluA2-containing #AMPAR purification from brain (30 min instead of >6 hours) from Jumi Park and Eric Gouaux. Higher yield and some previously unseen subunit combinations - including 2, 3 or 4 TARP auxiliary proteins.
#iGluRs

onlinelibrary.wiley.com/doi/10.1002/...

Photo-proximity labeling in live primary neurons identifies an AMPA-receptor signal transducer in homeostatic synaptic plasticity Basu et al. introduce an antibody-guided photo-proximity labeling strategy to probe GluA1 protein complexes on live neurons. This chemical approach captures transient, activity-dependent interactions ...

Just a note, using an extracellular antibody conjugate to do proximity biotinylation on live cells (neat trick!) should not label intracellular targets.

Is it local photodamage at labelled sites or dying cells? (these possibilities are noted by the authors)

#iGluRs

www.cell.com/cell-chemica...

What a neat way to make gold particles of higher order. Looking forward to see the tomograms of excitatory synapses with multiple labels (and much more)! #iGluRs

Calcium and magnesium in the pore of NMDA receptors!

Lipids play an unexpected role. The hydration shells of Ca2+ and Mg2+ could be the difference between permeation and block. So much physiology in the energetics of H2O.

Very stimulating work! Congrats @lab-furukawa.bsky.social

#iglurs

Beautiful work. We love to see those single channels and the connection to these open channel structures is 🔥
#iGluRs

Nice to see these detailed views of my dear GluK2, and the progress in the last 20 years since the first structures.

But perhaps the most important experiment to do now is the single channel recording of the staircase activation, to see if subconductances are there.

#iGluRs

#iglurs more intermediates and another bona fide open state. Congrats to all.

Activation of kainate receptor GluK2–Neto2 complex - Nature Structural & Molecular Biology Gangwar et al. present cryo-electron microscopy structures of GluK2 kainate receptors with auxiliary subunit Neto2 and reveal the basis of receptor activation.

Kainate receptor modulation by Neto including an open state. Surprising, to me: I would never have imagined that Neto works like a membrane-tethered lectin (ConA). Auxiliary proteins influence dimer-dimer interactions, as we and others also showed in AMPARs.

#iGluRs

www.nature.com/articles/s41...

PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Interested in how calcium permeates #AMPAreceptors (or not)? Check out our latest collaboration with @hansunlab.bsky.social from @fmp-berlin.de, now online in its final form. Congrats to former students Florian, Johann and Ece!
@humboldtuni.bsky.social @dfg.de #iGluRs

www.pnas.org/doi/10.1073/...

The molecular infrastructure of glutamatergic synapses in the mammalian forebrain

What if the PSD wasn't a defining feature of glutamatergic synapses, but simply a sample prep artifact?

^^ one of the many ideas Peukes et al. put forward while going from from live mice to cryo-CLEM/ET in <2 minutes #neuroskyence #iGluRs #cryoET

elifesciences.org/reviewed-pre...

Optochemical profiling of NMDAR molecular diversity at synaptic and extrasynaptic sites | The EMBO Journal imageimageNMDA receptors (NMDARs) play key roles in brain development and plasticity, and exist as multiple subtypes, whose individual characterization remains challenging in native tissues. This stud...

I am happy to announce the publication of our latest work on #photopharma of GluN2B NMDA receptors: www.embopress.org/doi/full/10..... I am very grateful to all the authors for their involvement in the work (@cecilecharrier.bsky.social, @cecidoso.bsky.social and all the blueskyless people). #iGluRs

AMPAR inhibition lowers S1P in human blood and improves murine autoimmune neuroinflammation by blocking T cell egress from lymph nodes A clinically available AMPAR antagonist inhibits T cell egress from lymph nodes and shows therapeutic effects in mouse models of multiple sclerosis.

Interesting results. Perampanel (AMPA blocker approved for epilepsy) has a big effect on T-cell mobilisation.

Perhaps an off-target effect? Maybe I missed a KO control using the floxed GluA123 mouse.

But useful either way, given that Pmp is quite mild.

#iGluRs

www.science.org/doi/epdf/10....

Here's our new preprint led by @wdhale.bsky.social with @rhuganir.bsky.social:

"Architecture, Activation, and Conformational Plasticity in the GluA4 AMPA Receptor"

www.biorxiv.org/content/10.1...

#iGluRs #cryoEM

Some key points below that Dylan discovered:

Super paper. Temperature shock has interesting implications for structure solution in general.
#glutamate #iglurs

Awesome.

The protein is an early bacterial clamshell domain with galactose (2GBP, from 1988!!!) from the master, Florante Quiocho. A prokaryotic prelude to understanding #iGluRs through #StructuralBiology and #Biophysics

www.science.org/doi/10.1126/...

The lab does #BPS2025! We loved showcasing our new work on #iGluRs. #proudPI

Awesome to meet up with old friends at #BPS2025
#iGluRs

Happy Valentines Day to all friends and colleagues in #MyVillage
#science #biophysics #iGluRs #AAAS2025 #BPS2025
NMDA receptor and much more...

Interested in #iGluRs? See below!

BPS2025 Los Angeles - Know Before You Go

#BPS2025 is so close!
Make sure you are well prepared.

Downlod the BPS Mobile App and use the Desktop Planner to develop an itinerary that misses none of the exciting opportunities of an in-person meeting.

@biophysicalsoc.bsky.social #science #ionchannels #iGluRs

mailchi.mp/biophysics/p...

Nice paper. I recommend to check the review file for a lot of extra interesting details. Still a lot to understand about TARP action, despite >20 years of work from many groups.
#iglurs #biophysics #receptors

You want to see kainate and forget AMPA? GYKI 53655 is still your best bet.

Unfortunately if you use a lot, you will also start to hit some kainate. It's not terrifically selective. But at 30 µM you should be fine.
www.cell.com/neuron/pdf/0...

Interested to see other answers!

#iGluRs

#iGluRs

Downstream of #iGluRs!

Proud moment today for many! We gathered to celebrate #science #NMDA receptors #iGluRs and the most recent doctor of phylosophy: Dr. Mae G. Weaver!