Original post on mastodon.green

Dear Nanopore folks, could you give an advice about an issue that my lab has with direct RNA sequencing on MinION. We're doing RNA004 sequencing of various samples and again and again, there's a staggering amount of reads with just repetitive AAG triplet. This happens for weird IVT RNA and […]

[Open Access]
Nanoscale direct patterning in ultrathin silicon nanosheets utilizing helium ion microscopy
2025 Jpn. J. Appl. Phys. 64 03SP57

iopscience.iop.org/article/10.3...

#JJAP
#Physics
#Openaccess
#Helium
#ion
#microscopy
#silicon
#nanosheet
#nanofabrication
#nanopore

🧬 Next @denbi.bsky.social training course: Nanopore Sequencing 101 🧬

Master ONT from library prep to data analysis! 🚀

📍 Bielefeld University
📅 30 June - 2 July
💻 Hands-on: Linux, Assembly, Data Analysis

Perfect for PhDs & Postdocs! 🔬

Read more: www.denbi.de/training-cou...

#Genomics #Nanopore

Scientists find antimicrobial-resistant genes on deep-sea mountains in the Arabian Sea | Research Matters New DNA sequencing of deep-sea sediments reveals a surprisingly diverse world of microbes that hold clues to global nutrient cycles and natural antibiotic resistance.

Scientists are using forensic eDNA to solve the mysteries of the deep-sea floor. By testing a scoop of dirt from the ocean floor, they’ve mapped entire ecosystems of bacteria and fungi.

To read more;
researchmatters.in/news/scienti...

#seamounts #DNAtech #microbes #nanopore #scicomm #science

Poster illustrating an upcoming webinar: PlasmidGo! Beginner’s Guide: ONT-based Whole Plasmid Sequencing. It features a headshot photo of our Product Marketing Manager Dr Nicole Ball, and details of the event, which is on Thursday 2nd April 2026 at 1000 GMT.

🧬 Webinar | Apr 2, 10 AM BST

Plasmid validation is evolving - are you?

Join Dr Nicole Ball to learn how ONT long-read sequencing enables full plasmid coverage (no more primer walking 👀)

tinyurl.com/5bj9xafz

#Plasmid #Genomics #Sequencing #Nanopore #SyntheticBiology #Biotech

Detection of DNA base modification using nanopore sequencing 2026 In this comprehensive two-day course, participants will learn how to use nanopore sequencing to detect DNA base modifications.

💬 "The most valuable part was the opportunity to bring my questions to the table and get insightful answers from the experts organising the course."

Develop your skills to detect, visualise, and interpret DNA base modification profiles across different contexts using #nanopore sequencing. 💻🧬

"The final library was loaded on a PromethION RNA flow cell (FLO-PRO004RA) and sequenced on a PromethION P2 solo"

#Nanopore #DiscontinuedSequencers

A nanopore-based HIV-1 reference epitranscriptome Abstract. Post-transcriptional modifications to RNA, which comprise the epitranscriptome, play important roles in RNA metabolism, gene regulation, and dise

New paper in *NAR*! 🧬
Nanopore-based HIV-1 epitranscriptome

Maps RNA modifications across full-length HIV RNAs → a field benchmark, including PLWH samples

Congrats to Li Zhang (co-first) from our lab, the Gagnon lab, and the whole team👏

🔗 tinyurl.com/3zup7t6t
#HIV #Epitranscriptomics #Nanopore

"an Oxford Nanopore Technologies (ONT, Oxford, UK) P2 solo sequencer"

#Nanopore #DiscontinuedSequencers

London Calling 2026 is less than months away! Discover our exciting agenda with a wide range of speakers sharing innovative approaches and unlocking novel biology with Oxford #Nanopore technology. #Nanoporeconf

Learn more here: https://bit.ly/46VeB85

💬 "Our aim is to help researchers make the most of real-time #nanopore #sequencing by providing an accessible, flexible, and open-source framework for metagenomic analysis."

Discover how software tool MARTi powers real-time classification and visualisation of #metagenomics. 💻 🧬

👉 buff.ly/iurkZDC

Comprehensive guide to maximise #nanopore #sequencing performance, covering experimental optimisation and advanced computational analysis to unlock richer #genomic and transcriptomic insights🧬🔬
📄 https://doi.org/10.1093/nar/gkag023
👤 EVBC: Sebastian Krautwurst, Martin Hölzer, Jörg Linde, Manja Marz

GW: ultra-fast chromosome-scale visualization of genomics data - Nature Methods Nature Methods - GW: ultra-fast chromosome-scale visualization of genomics data

🧬 GW — a new ultra-fast genome browser for chromosome-scale visualisation in @natmethods.nature.com
Unlike IGV, it handles megabase-scale regions with ease.
SV analysis with long-read sequencing! 🔬
nature.com/articles/s41592-025-02749-5
#Genomics #Nanopore #StructuralVariants

Learn more about adaptive sampling in our latest #Nanopore Know-How: nanoporetech.com/blog/guided-by-data-adap... #ACMGtg26

Proud that this work is now published!

We hope it helps orient future #Nanopore long-read sequencing studies hunting for structural variants

Also really happy to share eight new high-coverage (>100x) long-read read pools and assemblies with the #Drosophila community!

doi.org/10.1093/g3jo...

Last call for these 2 different #PhD positions in #nanopore tech:
🗓️ apply by March 15 to join our team at the nano-dynamics group at @unibas.ch Switzerland.
Follow the guidelines online: schmid.chemie.unibas.ch/en/join-us-1/

Mitotic microhomology-mediated break-induced replication promotes chromoanasynthesis - Nature Communications Chromoanasynthesis is an elusive form of complex chromosomal rearrangement commonly detected in cancer and congenital disorder. Here, the authors develop a long-read DNA sequencing approach and show t...

Excited to share our new paper in @NatureComms!
Using #nanopore sequencing, we uncover how mitotic microhomology-mediated break-induced replication #MMBIR can generate #chromoanasynthesis / complex genome rearrangements linked to cancer and congenital disorders.
www.nature.com/articles/s41...

Hannah

We are delighted to welcome Hannah to our group. She will conduct long-read sequencing to explore plant genomes. We are excited to have her on board and look forward to amazing discoveries ahead!

www.izmb.uni-bonn.de/en/pbb/team

#PlantGenomics #LongReadSequencing #Bioinformatics #Nanopore

"We found that the elevated read counts and sequencing depth generated by Illumina NGS were offset by the greater read length obtained on the MinION MK1C and resulted in comparable pathogen genome coverage between the two platforms."

#Nanopore #DiscontinuedSequencers

𝘼𝙗 𝙞𝙣𝙞𝙩𝙞𝙤 𝙙𝙚𝙩𝙚𝙘𝙩𝙞𝙤𝙣 𝙤𝙛 𝙢𝙪𝙡𝙩𝙞𝙥𝙡𝙚 𝙚𝙥𝙞𝙩𝙧𝙖𝙣𝙨𝙘𝙧𝙞𝙥𝙩𝙤𝙢𝙞𝙘 𝙢𝙤𝙙𝙞𝙛𝙞𝙘𝙖𝙩𝙞𝙤𝙣𝙨 𝙛𝙧𝙤𝙢 𝙊𝙉𝙏 𝙙𝙞𝙧𝙚𝙘𝙩 𝙍𝙉𝘼 𝙨𝙚𝙦𝙪𝙚𝙣𝙘𝙞𝙣𝙜 𝙙𝙖𝙩𝙖

🔗 pubmed.ncbi.nlm.nih.gov/41603648/

#Nanopore #Bioinformatics
@nanoporetech.com

New #OpenAccess research in #RESMedVetEnt

A multiplex #assay to detect #mosquito species, bloodmeal host source and #Plasmodium in #malaria vectors using #Nanopore amplicon #sequencing
doi.org/10.1111/mve.70055

#EntoMethods #InsectVectors #InsectBorneDiseases
@wileyecology.bsky.social

Detection of DNA base modification using nanopore sequencing 2026 In this comprehensive two-day course, participants will learn how to use nanopore sequencing to detect DNA base modifications.

✅️ Registration is open for our Detection of DNA base modification using #nanopore #sequencing. We'll be covering experimental design, considerations for ultra-#longreads, visualisation of data, and comparison of approaches. 🧬💻

📍 Online (via Zoom)
📅 Register by: 10 April

🧬 #NARGAB New Research Spotlight
Direct #RNA #nanopore sequencing reveals the most detailed #m6A map to date across Human immunodeficiency virus 1 transcripts #HIV-1. @cnrs.fr @i2bcparissaclay.bsky.social
👉 Read the full paper: doi.org/10.1093/narg...

But at the moment, @nanoporetech.com needs to make new clair3 models in pytorch for #SUP version 5.2.0 with move table support #nanopore

www.biorxiv.org/content/10.6... Clair3 v2 for #nanopore reads, it keeps getting better!

Anyone has tips to better balance the reads between barcodes with the @nanoporetech.com rapid barcoding kits? With the exception of 2 all these samples had an input of +/-200ng. There could ofc be small quantification/pippete errors but the difference between samples are sometimes huge. #nanopore

A new NAR Genomics and Bioinformatics resource introduces SquiDBase, a community repository of raw #nanopore #microbiome sequencing data, supporting open access and broader utility for bioinformatics and comparative analyses. #Genomics
📄 https://doi.org/10.1093/nargab/lqaf213
👤 EVBC: Daan Jansen

Learn more about adaptive sampling in our latest #Nanopore Know-How. Learn more here: nanoporetech.com/blog/guided-by-data-adap...

Defining expansions and perturbations to the RNA polymerase III transcriptome and epitranscriptome by modified direct RNA nanopore sequencing - Nature Communications RNA polymerase III transcribes essential non-coding RNAs, but many aspects of this biology remain unclear. Here, the authors develop DRAP3R, a nanopore sequencing method that captures Pol III transcripts and RNA modifications, revealing new RNAs and dynamic modification patterns.

New paper uses a modified direct #RNA #nanopore sequencing framework (DRAP3R) to reveal expanded RNA polymerase III #transcriptome and epitranscriptome landscapes, including novel Pol III-transcribed RNAs. 🧬
📄 https://doi.org/10.1038/s41467-025-68230-1
👤 EVBC member: Daniel Depledge

Forgot to tag #nanopore if anyone has that tag in his/her feeds