#WIS

#PlantandEnvironmentalScience

#RibonucleicAcid

#RNAStability

#TranslationEfficiency

#Pseudouridine

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🚨 New paper published in RNA!
Scientists often say anecdotally that RNA modifications are disrupted in immortalized cells — but no one’s really tested it.
So we did: ψ-mapping in primary T cells vs. Jurkat cells using direct RNA-seq.
📄 tinyurl.com/TCellPsiRNA
#nanopore #RNA #pseudouridine

Pseudouridine RNA avoids immune detection through impaired endolysosomal processing and TLR engagement Toll-like receptors (TLRs) 7 and 8 detect RNA degradation products to sense infection. However, modified nucleosides common in self-RNA—most notably pseudouridine—evade recognition. This study finds t...

#Pseudouridine #RNA avoids immune detection through impaired endolysosomal processing and TLR engagement: Cell www.cell.com/cell/fulltex...

Proud to be part of this interdisciplinary work with @sebastianglatt.bsky.social, Ting-Yu Lin, our teams at @MCB_UJ & @IIMCB_Warsaw, and collaborators from France! #cryoEM + #biophysics + #modeling reveal how #pseudouridine shapes human tRNA structure and stability. 🧬 #RNAmodifications

tRNAs are small and sport heterogeneous base modifications – yet @sebastianglatt.bsky.social @jmbujnicki.bsky.social Ting-Yu Lin 林亭妤 et al show #cryoEM combined with biophysical & computational approaches can give insights into how #pseudouridine affects human tRNA!
www.embopress.org/doi/full/10....

Did you know? The #ViennaRNA Package can handle a wide range of RNA modifications, including:

#Inosine (I)
#Pseudouridine (P)
#N6-methyladenosine (m6A)
#7-deaza-adenosine (7DA)
#Nebularine (purine)
#Dihydrouridine (D)

#Bioinformatics #ComputationalBiology #RNAStructure #SyntheticBiology
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