hPSC-derived #CardiacOrganoids (Ventricular Cardiomyocyte + Epicardial cell)

Modeling #CardiacFibroblast heterogeneity during Cardiac injury (isoproterenol/hypoxia/TGFβ1)➡️
7 reparative + 5 non-reparative fibroblast states

#NatureComms 2023
www.nature.com/articles/s41...

scRNAseq + #SpatialTranscriptomics #Visium #MyocardialInfarction

#CardiacFibroblast (HTRA3-TGFβ) Paracine ➡️#Cardiomyocyte (Nox4, DNA damage, IGFBP7) #Senescence #Degeneration

Intriguing evidence👉TGFβ drives DNA damage accumulation🤠

#NatureComms 2022
www.nature.com/articles/s41...

#MatrixStiffness #Senescence

Passage-dependent senescent responses of cultured #CardiacFibroblast to Stiffness

Stiff hydrogel: 41 kPa
Soft: 6 kPa

Soft matrix⏫senescence (P2), ⏬TGFβ & ⏫NFκB-SASP (P5-6)

Timothy McKinsey lab #AJPHeartCirc 2023
journals.physiology.org/doi/abs/10.1...

#BMP9 #MyocardialInfarction

BMP9 expressed by #EndothelialCell #CardiacFibroblast Not #Cardiomyocyte

BMP9 KO🐭post-MI->
⏬Survival
⏫TGFβ signal, #CardiacFibrosis, MMP9

How about #Angiogenesis/#EndMT?🧐

Navin Kapur lab #JACCBTS 2023
www.sciencedirect.com/science/arti...