To identify novel regulators of the #MDA5 pathway, we used SILAC-MS in #virus infected vs uninfected cells. We identified ~100 MDA5 binding partners, one of which was Annexin A2 (ANXA2).

🚨 Preprint Alert 🚨

We discovered a new regulator of RIG-I-like receptors #MDA5 #RIG-I, which are critical innate immune sensors of #RNA that detect #viral infection.

Check out our #biorxiv #preprint: www.biorxiv.org/content/10.1...

tl;dr 🙃 thread below

Super fun presenting some of our #MDA5 #RNA sensor work to an RNA-centric audience! The different point of view from my usual infection and immunity background led to some great questions and discussions. More ideas forming!!! #ARNASydney2025 #virus 🧪🧫🦠

A LncRNA-Encoded Interferon Memory Circuit Mediated by MDA5 Type I interferon (IFN) signaling is central to antiviral immunity and immune homeostasis, yet the mechanisms that sustain and amplify this response remain unclear. Here, we identify two IFN-inducible...

First post on Bluesky—
We identify two IFN-inducible lncRNAs as endogenous MDA5 ligands forming an RNA–sensor feedback loop that sustains innate immunity.
m⁵C + ALY/REF = cytoplasmic targeting switch

Read more www.researchsquare.com/article/rs-6...

#InnateImmunity #lncRNA #MDA5 #Autoimmunity

Of course, I wonder, what #MDA5 is doing in all of this? But maybe thats for another paper... Anyway, a great article, I highly recommend this to the #virus #RNA #innateimmunity #RIGI #RLR peoples!

#MDA5 AB+
#Sjoerns
#RA
##AtropicGastritis

This dsRNA is likely from aberrant transcription of non-exonic RNA in/near ISGs, often introns that contain repetitive elements - sequences known to form double-stranded structures that activate dsRNA sensors like #MDA5 and #PKR.

Such a tour de force paper, a really nice read!

Wow what an amazing article! Very cool to see the important role of #macrophages and #interferon in the #tumour microenvironment. I would bet 💵 that the IFN-driving azacitidine works via #MDA5, as this has been shown to activate MDA5 via ERV expression in cancer cells 😄!

GGNBP2 regulates MDA5 sensing triggered by self double-stranded RNA following loss of ADAR1 editing GGNBP2, CNOT10, and CNOT11 are required for innate immune response after the loss of ADAR1-mediated editing of self dsRNA.

This week in #ScienceImmunology: @jackiheraudfarlow.bsky.social , Carl Walkley, & colleagues used a genome-wide #CRISPRscreen to determine that a GGNBP2– CNOT10/11 complex is required for the accumulation of unedited #immunogenic self- #dsRNA & its sensing by #MDA5!

Newfound autoimmune syndrome tied to COVID-19 can trigger deadly lung scarring A surge in cases of a rare autoimmune disease during COVID-19 waves in England led to the discovery of a new syndrome.

Newfound #autoimmune syndrome caused by #COVID19 triggers lethal lung scarring MDA5-autoimmunity & interstitial pneumonitis contemporaneous with COVID-19 #Pandemic or MIP-C . #MDA5 dermatomyositis occurs when immune system attacks its own MDA5 proteins.
www.livescience.com/health/coron...

COVID-19 linked to rise in autoimmune lung disease, study finds A study in eBioMedicine identifies shared immunopathology between SARS-CoV-2 infection and MDA5 autoimmunity, highlighting a surge in MDA5+ cases and interstitial lung disease during the COVID-19 pand...

COVID-19 linked to rise in autoimmune lung disease, study finds 🦠🫁🔬 www.news-medical.net/news/2024051... #Autoimmunity #COVID19 #LungDisease #Immunology #MDA5 #eBioMedicine #Research #PublicHealth #Pandemic

Through TLR3, ZIKV induces proinflammatory #cytokines (IL-6, IL-10), which trigger STAT3 signaling, which in turn dampen #interferon responses. As #RIGI and #MDA5 sense ZIKV and induce IFN production, this antagonism eventually aids viral replication as we can show.