Map-based cloning of CF site. (A) The Δ(SNP-index) value on chromosomes in reference genome ‘ZS11’ through MutMap. Only Chromosome C05 had a peak above the threshold. (B) The CF locus was delimited to a 52.21-kb interval between two flanking markers, S43.81 and S43.86, using eight recombinant plants with extreme phenotypes from 4600 F2 individuals. (C) Annotated genes in the CF locus. Bar = 1 kb. The red letter indicates the most likely candidate gene for CF. (D) Gene structure and mutation sites of BnaC05.POLIB, including nucleotide substitutions in WT and cf. A substitution mutation (G to A) was identified at nucleotide position 3128. Bar, 100 bp.

#Rapeseed chimeric flower

BnaC05.POLIB exon 7: G→A (D742N) → Mg²⁺ disruption → #polymerase ↓83%

Plastid genome copies ↓ (white sectors) → #chromoplast defects → carotenoids ↓78%

Dosage: het = 30-50% white; hom = 60-80% + growth defects

Map-based cloning + #CRISPR ✓

doi.org/10.1093/hr/u...

Isothermal DNA Amplification or LAMP is sensitive, robust, requires no prior experience & can be performed in 30 minutes. 
Convert today with expert LAMP enzymes & master mixes from Jena Bioscience

🔗www.stratech.co.uk/jena_bioscience/isotherm...

#PCR #Polymerase #DNA

Validate User

A bit less recent work published in @narjournal.bsky.social led by Klein, Das and Bera. Great collaboration with many labs showing that SARS-CoV-2 polymerase must undergo an activation step to enable RNA synthesis.
doi.org/10.1093/nar/...
#SARSCoV2 #polymerase #RNAvirus #singlemolecule

Mechanism of co-transcriptional cap snatching by #influenza #polymerase

Mechanism of co-transcriptional cap snatching by #influenza #polymerase, etidiohnew.blogspot.com/2026/03/mech...

1/9
🦠 ⚠️ H5N1 ADAPTING TO MAMMALS: NEW GENETIC CLUES ⚠️🦠

A new study reveals how the #H5N1 "bird flu" virus has evolved to replicate in dairy cattle.

Key mutations in the virus's #Polymerase are the smoking gun.

Nature reports...
#Virology #PublicHealth #BirdFlu #AvianFlu #PandemicPreparedness

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals, etidiohnew.blogspot.com/2026/01/poly...

CryoSiam: self-supervised representation learning for automated analysis of cryo-electron tomograms Cryo-electron tomography (cryo-ET) enables visualization of macromolecular complexes in their native cellular context, but interpretation remains challenging due to high noise levels, missing informat...

Another beautiful example of new methods for biological discovery: #CryoSIAM is an embedding representation framework that can denoise, segment and pick particles – which the authors then used to pick RNA #polymerase in the Chlamy dataset!

The Traveler, by Polymerase from the album Unostentatious

🇺🇦 #NowPlaying on #KEXP's #AstralPlane

Polymerase:
🎵 The Traveler

#Polymerase

▶️ 🪄 Automagic 🔊 show 📻 playlist on Spotify

▶️ Song/Cover on #Bandcamp:

Emergence of #mammalian-adaptive #PB2 #mutations enhances #polymerase activity and #pathogenicity of #cattle-derived #H5N1 #influenza A virus

Emergence of #mammalian-adaptive #PB2 #mutations enhances #polymerase activity and #pathogenicity of #cattle-derived #H5N1 #influenza A virus, etidiohnew.blogspot.com/2025/12/emer...

An evolutionary approach to identify #mammalian adaptive #mutations in the avian #influenza #polymerase complex

An evolutionary approach to identify #mammalian adaptive #mutations in the avian #influenza #polymerase complex, etidiohnew.blogspot.com/2025/10/an-e...

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals.

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals, etidiohnew.blogspot.com/2025/08/poly...

Subculture: After Dark Ep #035 David Griffiths and Kyle McGrath are back with a new episode of Subculture: After Dark. This time around they chat to A Skylit Drive, ESA, Fit For A King, Polymerase, Wormrot, Pig Destroyer, Combichrist and Ill Nino.

📣 New Podcast! "Subculture: After Dark Ep #035" on @Spreaker #askylitdrive #combichrist #davidgriffiths #esa #fitforaking #harleywoods #illnino #pigdestroyer #polymerase #subcultureafterdark #wormrot

POLYMERASE - Mindspace Interview Polymerase chat to Subculture about the new Mindspace album.

📣 New Podcast! "POLYMERASE - Mindspace Interview" on @Spreaker #davidgriffiths #polymerase #subcultureafterdark

Novel open-source diagnostic tool offers affordable, reliable pathogen detection for resource-limited settings A bottleneck in ensuring access to widespread molecular diagnostics, especially in low- and middle-income countries, has been the high cost and logistical complexities associated with rapid,…

Open-source #diagnostic tool offers affordable, reliable #pathogen detection for resource-limited settings ...

| #lyophilization | #DNA | #polymerase | #glycosylase | #Ecoli | By @rupress.org via @sciencex.bsky.social

[News] Filipino band Polymerase explore transcendence and spiritual awakening on new album "Mindspace" The Filipino Psychedelic Doom Metal band Polymerase released their new album “Mindspace” on May 14th. Available digitally via POT Arrow Records, the album is sure to captivate fans of Elder, Sleep, Om...

[News] Filipino band Polymerase explore transcendence and spiritual awakening on new album "Mindspace"

progrockjournal.com/news-filipin...

#progrockjournal #polymerase #newrelease #newalbum #stoner #doom #heavymusic #sludge #heavymetal #music

A portable low-cost #Arduino-controlled #OpenSource #polymerase chain reaction device:

-4-well aluminum heating block
-heated lid
-heating/cooling rates: 1.78/1.52 °C/s
-temp. accuracy: ± 0.55 °C
-cost: US $120
-requires #MATLAB

doi.org/10.1016/j.oh...
#DIYbio #lab #instruments #PCR #DNA

#Influenza A virus #polymerase co-opts distinct sets of host proteins for #RNA transcription or #replication

#Influenza A virus #polymerase co-opts distinct sets of host proteins for #RNA transcription or #replication, etidiohnew.blogspot.com/2025/06/infl...

From disorder to order: cryo-EM reveals RNA-dependent remodeling of Nipah virus polymerase A flurry of recent structural studies have focused on the polymerase complex of the deadly zoonotic pathogen Nipah virus (NiV). These include a report…

My PhD student @rupeshjayachandran.bsky.social wrote a comment piece on recent #nipah virus #polymerase structures, especially the one reported by @salafernanda.bsky.social from the lab of @haukehillen.bsky.social

Please check it out 😊

www.sciencedirect.com/science/arti...

@science.org 🧫🧬❄️🔬 Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis | Science www.science.org/doi/10.1126/...
@yiweichang.bsky.social www.yiweichanglab.org @jiwasa.bsky.social #virology #Influenza #Cryo-EM #StructuralBiology #RNA #polymerase

Isothermal DNA Amplification or LAMP is sensitive, robust, requires no prior experience and can be performed in 30 minutes. Convert today with expert LAMP enzymes and master mixes from #JenaBioscience
Learn more here: www.stratech.co.uk/jena_bioscie...

#PCR #Polymerase #DNA

So many life processes remind me of #DNA #polymerase.

Like, no single step really can be completed in isolation: once #script has been laid down in pen, if we hit a wall, I need to go back to the keyboard.

And presumably once I'm #recording, I'm going to have to be returning to the #pen.
#writing

Check out the latest NAR #Breakthrough article on directed enzyme evolution to re-engineer a terminal DNA polymerase for commercial DNA synthesis #enzyme #engineering #polymerase #DNAsynthesis 👉 read the full article here: https://doi.org/10.1093/nar/gkaf115

Optical tweezers reveal MoA of 3 small-molecule inhibitor classes against #tuberculosis #RNA #polymerase, suggesting combining two of the three antibiotics (Stl & D-IX216) to induce complete transcription arrest and therapeutic effect.

Akshaya Seshadri and Anjana Badrinarayanan @anjbadri.bsky.social @ncbsbangalore.bsky.social @tifrscience.bsky.social show that exonuclease action of replicative #polymerase gamma drives damage-induced #mitochondria #DNA clearance. New mechanism of mtDNA clearance.

www.embopress.org/doi/full/10....

#DYK that this method is mainly based on #Polymerase Chain Reaction (PCR) techniques?

#Darwin_glossary #FoodSafety

Here is our November issue.

embopress.org/toc/14693178...

The cover features work by George Gao and co-workers showing that NS2 induces an #influenza A RNA #polymerase hexamer and acts as a #transcription to #replication switch.

-> embopress.org/doi/full/10....

5/n In addition to the above shown examples for #MolecularMachines, here a few more #molecules we presented in #AugmentedReality at #IdeenExpo: #Tubulin-stathmin complex, #antibody, #nucleosome particle, #SARSCoV2 #polymerase.
#DIYbio #MINT #STEM #chem #AR #education #TiltFive