#ProteinTagging hashtag - Bluesky

@promegauk.bsky.social

5 months ago

Tomorrow (22 Oct 2025) at 7.00pm (BST): Free webinar - Generating CRISPR Knock-in Cell Lines - practical guidance on tag choice, knock-in design, and validation with Promega scientists Dr. Marie Schwinn and Dr. Chris Eggers.

Register: https://tinyurl.com/22Oct2025Webinar

#CRISPR #ProteinTagging

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Promega UK

@promegauk.bsky.social

5 months ago

Free webinar: Generating CRISPR Knock-in Cell Lines

🗓 Wed 22 Oct, 7pm BST
Promega scientists Dr. Marie Schwinn & Dr. Chris Eggers share guidance on choosing tags, designing knock-ins, and validating endogenous protein fusions.

Register: https://tinyurl.com/22Oct2025Webinar

#CRISPR #ProteinTagging

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Promega UK

@promegauk.bsky.social

8 months ago

Reminder: Our CRISPR protein tagging webinar is tomorrow (16 July, 3.00 p.m. BST).

Learn how to design and validate knock-in tagging workflows with HiBiT, NanoLuc® and HaloTag®.

Register at: http://bit.ly/4lDuRQ9

#CRISPR #ProteinTagging

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Promega UK

@promegauk.bsky.social

8 months ago

Free webinar – 16 July at 3.00 p.m. BST
Learn how to apply CRISPR-based protein tagging using HiBiT, NanoLuc® or HaloTag®. Includes Q&A with Promega experts.

Register at: http://bit.ly/4lDuRQ9

#CRISPR #ProteinTagging

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UPENN_ITMAT

@itmat.bsky.social

10 months ago

Intracellular protein editing enables incorporation of noncanonical residues in endogenous proteins The ability to study proteins in their native cellular context is crucial to our understanding of biology. In this work, we report a technology for intracellular protein editing, drawing from split intein–mediated protein splicing, genetic code expansion,...

Expanding the toolbox for #ProteinTagging: #Posttranslational #ProteinEditing in living #MammalianCells allows classic cell biology experiments while retaining #ProteinFunction www.science.org/doi/10.1126/... @BurslemLab #TranslationalScience

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GenomeTDCC

@genometdcc.org

1 year ago

High-Throughput Tagging of Endogenous Loci for Rapid Characterization of Protein Function Alejandro Chavez, MD, PhD, and Joonwon Kim, PhD of UCSD discuss their recent *Science Advances* publication on high-throughput insertion of tags across the genome (HITAG). This cutting-edge technique enables rapid and efficient tagging of endogenous proteins, providing an invaluable tool for the large-scale interrogation of protein function. *What is HITAG?* HITAG (High-Throughput Insertion of Tags Across the Genome) is a novel method designed to facilitate the study of protein function on a large scale. By leveraging a modified Cas9-based targeted insertion strategy that relies on nonhomologous end joining (NHEJ), HITAG allows for the rapid creation of libraries of cells, each containing a different protein of interest tagged at the C-terminus. *Applications:* Using HITAG, the researchers fused mCherry to a set of 167 stress granule-associated proteins. This enabled them to elucidate the features driving a subset of proteins to accumulate strongly within these transient RNA-protein granules. *Why is HITAG Important?* Understanding the dynamic behavior and interaction partners of proteins is crucial for building an accurate working model of the cell. Protein tags, such as those created by HITAG, facilitate numerous studies, including in vivo protein localization, affinity purification, and rapid protein degradation. *Read the full article in Science Advances:* https://www.science.org/doi/10.1126/sciadv.adg8771 *Plasmid constructs available at the nonprofit repository, Addgene* https://www.addgene.org/browse/article/28238585/ *Learn more about the Chavez Lab and research interests:* https://chavezlab.com/ Discover more about the *NHGRI Opportunity Funds* and the research supported through the NHGRI Genome Technology Program: https://genometdcc.org/opportunity-fund-program/

🧪🧬🖥️
Dive into the future of protein research with HITAG from Alejandro Chavez+Joonwon Kim et al! Discover how this revolutionary technique enables rapid and scalable tagging of proteins for comprehensive function analysis.
www.youtube.com/watch?v=boGW...
🔬 #ProteinTagging #HITAG #CRISPR #STS

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