Milo B. Fasken, Ph.D.

@milofasken.bsky.social

3 months ago

The RNA exosome maintains cellular RNA homeostasis by controlling transcript abundance in the brain Higginson et al. present a cell-type-resolved atlas of RNA exosome dysfunction in the Drosophila brain, showing that pathogenic Rrp40 mutations disrupt RNA surveillance and neuronal integrity. This re...

Exollent! Congrats!
#RNAExosome #RNA

www.cell.com/cell-reports...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

3 months ago

Nuclear exosome targeting complex safeguards hematopoietic stem cell self-renewal and genomic integrity through resolving R loops R loops form during transcription, threatening the genomic stability of hematopoietic stem cells (HSCs) and lymphoma cells. Pan et al. report that ZCCHC8 safeguards the genome integrity and self-renew...

#NEXT story to read! Cheers!
#RNAExosome
www.cell.com/cell-reports...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

4 months ago

DIS3 licenses B cells for plasma cell differentiation in humans - Cellular & Molecular Immunology Cellular & Molecular Immunology - DIS3 licenses B cells for plasma cell differentiation in humans

Look at DIS! #DIS3 #RNAExosome #PlasmaCells

www.nature.com/articles/s41...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

5 months ago

Congrats! #SSUprocessome #RNAExosome #rRNA

www.nature.com/articles/s41...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

5 months ago

EXOSC10 haploinsufficiency causes primary microcephaly by derepression of Sonic hedgehog signalling Ulmke et al. show that mutations in EXOSC10 cause microcephaly. In mice, loss of Exosc10 leads to premature differentiation of neural progenitor cells, dis

#EXOSC10 #RNAExosome #RNA

academic.oup.com/brain/advanc...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

5 months ago

Helicase-mediated mechanism of SSU processome maturation and disassembly Eukaryotic ribosomal small subunit (SSU) assembly requires the SSU processome, a nucleolar precursor containing the RNA chaperone U3 snoRNA. The underlying molecular mechanisms of SSU processome matur...

Tour de force stuff!

#SSU #Processome #RNAExosome

www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

7 months ago

4D structural biology–quantitative dynamics in the eukaryotic RNA exosome complex - Nature Communications An integrated structural biology approach combining NMR, cryo-EM, X-ray crystallography and molecular dynamics simulations is implemented to characterise the conformational dynamics and interactions o...

Cool!
#RNAExosome #RNA #RNAsky

www.nature.com/articles/s41...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

8 months ago

Cellular imbalance of specific RNA binding proteins associates with harmful R-loops Author summary R-loops are three-stranded structures formed by a DNA-RNA hybrid and the displaced ssDNA. The accumulation of R-loops damages the genome, highlighting the need for their tight regulatio...

#Dis3 #Rloop #RBP #RNAExosome #RNA

journals.plos.org/plosgenetics...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

9 months ago

Interesting! #RNAExosome #RNA
#EXOSC10 -mediated pre- #tRNA #surveillance safeguards #neuron survival
Yoshinori Nishimoto, Xin Yang, Richard I. Gregory

www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

9 months ago

RNA Dynamics Regulate Transcriptional Condensate Vivacity to Drive Gene Coordination Transcriptional condensates (TCs), enriched with Mediator, orchestrate super-enhancer (SE)-driven gene expression critical for cell identity. However, how their dynamic physical properties shape trans...

#RNA #Condensate #RNAExosome #RNADecay

www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

9 months ago

Nice!
#RNAExosome #Structure #Review #RNADecay #RNA #RNAsky

www.annualreviews.org/content/jour...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

10 months ago

Inhibition of the nucleolar RNA exosome facilitates adaptation to starvation Cells reallocate their limited energy for cellular maintenance in response to nutrient scarcity at the expense of other processes, but how this trade-off is achieved remains unknown. These authors sho...

#RNAExosome #RNA #Starvation
doi.org/10.1371/jour...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

10 months ago

Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach - Nature Communications Human pathways acting on nuclear ncRNAs have been refractory to forward genetics. Here, the authors develop a forward genetic approach that identifies such pathways and show DDX59 is required for mino...

A tour de force. Wonderful stuff! Congrats!
#ncRNA #MALAT1 #RNAExosome #RNaseP #NEXT #DDX59 #RNADecay #RNA #RNAsky

www.nature.com/articles/s41...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

11 months ago

TRAMP assembly alters the conformation and RNA binding of Mtr4 and Trf4-Air2 | PNAS The TRAMP complex contains two enzymatic activities essential for RNA processing upstream of the nuclear exosome. Within TRAMP, RNA is 3′ polyadeny...

Nice work from Sean Johnson on "The Molecular Mechanisms of RNA Processing in the TRAMP RNA Surveillance Complex presented today at #RNASociety #Emory GA RNA Salon! @rnasociety.bsky.social #RNA #TRAMP #RNAExosome #RNADecay #Mtr4

www.pnas.org/doi/10.1073/...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

11 months ago

Aging-dependent dysregulation of EXOSC2 is maintained in cancer as a dependency Reprogramming of aged donor tissue cells into induced pluripotent stem cells (A-iPSC) preserved the epigenetic memory of aged-donor tissue, defined as genomic instability and poor tissue differentiati...

Interesting!
#RNAExosome #EXOSC2 #RNADecay #RNAprocessing #Aging #Cancer

www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Great work! #RNAExosome #Assembly #RNADecay #RNA #Mammalian

Hierarchical assembly and functional resilience of the mammalian RNA exosome
Tsimafei Navalayeu...Stefan L. Ameres
www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

The nuclear exosome co-factor MTR4 shapes the transcriptome for meiotic initiation Nature Communications - Nuclear RNA decay regulates gene expression in cultured cells, but its role in animals is largely unclear. Here, authors found that the nuclear exosome co-factor MTR4...

Cool! #MTR4 #RNADecay #RNA #RNAExosome
The #nuclear #exosome co-factor #MTR4 shapes the #transcriptome for #meiotic initiation

Li Zhang, Jianshu Wang, Zhidong Tang, Zhen Lin, Ruibao Su, Naijing Hu, Yao Tang, Gaoxiang Ge, Jing Fan, Ming-Han Tong, Yuanchao Xue, Yu Zhou & Hong Cheng
rdcu.be/ed5WM

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Defining the roles of the Integrator, NEXT, and nuclear exosome complexes in Drosophila oogenesis Nuclear RNA homeostasis depends on the balance of transcription, RNA processing, degradation, and transport between the nucleus and cytoplasm. RNA degradation directed by the Integrator, nuclear exoso...

Interesting! #RNADecay #RNAExosome #RNA #ncRNA

Defining the roles of the #Integrator, #NEXT, and #nuclear #exosome complexes in #Drosophila #oogenesis
Yongjin Lee, Adriano Biasini, Cindy Tipping, Seong Hyeon Hong, Phillip Zamore

www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

New Insights into nuclear import and nucleolar localization of yeast RNA exosome subunits The RNA exosome is a multiprotein complex essential for RNA maturation and degradation. In budding yeast, a nine-subunit protein complex (Exo9) associated with Rrp44 forms Exo10 in the cytoplasm and, ...

Nice! #RNAExosome #Localization #BuddingYeast

New Insights into #nuclear #import and #nucleolar #localization of yeast #RNA #exosome #subunits

Valdir Gomes Neto...Carla C. Oliveira
www.biorxiv.org/content/10.1...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

RNA exosome-driven RNA processing instructs the duration of the unfolded protein response Abstract. Upon stresses, cellular compartments initiate adaptive programs meant to restore homeostasis. Dedicated to the resolution of transient perturbati

#RNAExosome #Rrp6 #RNAProcessing #Splicing #UPR #UnfoldedProteinResponse #BuddingYeast

academic.oup.com/nar/article/...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Degradation of circular RNA by the ribonuclease DIS3 Features of circular RNAs (circRNAs) produced by back-splicing of eukaryotic exon(s) make them resistant to degradation by linear RNA decay machinerie…

DIS is interesting!
#circRNA #Degradation #DIS3 #Ribonuclease #RNAExosome

www.sciencedirect.com/science/arti...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

#DIS3L #RNAExosome #Development #Mouse #ProteinSynthesis #RNA

DIS3L, cytoplasmic exosome catalytic subunit, is essential for development but not cell viability in mice
Brouze et al.

m.rnajournal.cshlp.org/content/earl...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Mutant RNA exosome protein linked to neurodevelopmental defects Researchers at Emory University find that a missense mutation impairs RNA exosome assembly and translation and causes neurological disease.

Cheers for write-up, Meric Ozturk! Great job!
#RNAExosome #RNA #Neurodevelopmental #Disease

www.asbmb.org/asbmb-today/...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

Interesting.
#rRNA #60S #RNAExosome #RNA #RNAsky

Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis

www.sciencedirect.com/science/arti...

Milo B. Fasken, Ph.D.

@milofasken.bsky.social

1 year ago

RNA 3′end tailing safeguards cells against products of pervasive transcription termination - Nature Communications Pervasive transcription generates numerous unadenylated RNAs, usually degraded by the NEXT/nuclear exosome pathway. Here, the authors show that, upon NEXT inactivation, these RNAs are removed by compe...

Interesting! #RNADecay #NEXT #PAXT #RNAExosome #RNA
www.nature.com/articles/s41...