Immunoprecipitation & Western Blot Workshop Join us for this Immunoprecipitation & Western Blot Workshop and Learn from Proteintech - the Antibody Experts.

Join us at @DKFZ German Cancer Research Center in Heidelberg for our upcoming #Immunoprecipitation & #WesternBlot Workshop 🔬

📅 Tuesday, April 14
🕒 12:30–14:00

Free pizza, goodies, and a chance to chat with our team 🍕

www.eventbrite.co.uk/e/immunoprec...

Read the full study:
www.mdpi.com/2673-6772/6/...

#Toxoplasmosis #CongenitalToxoplasmosis #Diagnostics #WesternBlot #PublicHealth #Microbiology #Parasitology #LaboratoryMedicine #Surveillance #Science 6/6

Quantitative western blotting adapted for expanded sample capacity with non-linear curve fitting - Journal of Plant Research Journal of Plant Research - Quantification of cellular protein levels is a central aspect of molecular biology. Although large-scale proteomic quantification technologies have been developed,...

“Quantitative western blotting adapted for expanded sample capacity with non-linear curve fitting.” Cheng et al. #WesternBlot #ProteinQuantification link.springer.com/article/10.1...

Every biotech student must master these essential lab techniques!
From DNA extraction & PCR to Western blotting at BioResire.
Learn hands-on: info@bioresire.in
#Biotechnology #Biotech #MolecularBiology #PCR #DNAextraction #qPCR #GelElectrophoresis #WesternBlot #RNAPurification #RestrictionDigestion

🔬 Southern, Northern, Western Blot. Three techniques, one principle, the entire central dogma made visible. Here is why every aging researcher needs to understand all three.

#MolecularBiology #Techniques #WesternBlot #AgingResearch #Longevity #Genomics #Proteomics #LabScience #Hallmarks

❓Tech Q&A | Band misalignment in #WB is usually technical, not antibody-related.
👉Common causes:
• Uneven gel #polymerization from poor mixing
• Air bubbles in gel affecting migration
• Sample-buffer interference alters mobility
• Overloading samples
#WesternBlot #LabTroubleshooting

❓Tech Q&A | SR-18292 may fail to inhibit #PGC1α due to insufficient dose and short exposure.
🔎Reported studies use higher concentrations (~20 μM) and longer incubation
👉Try extending treatment time and expanding the dose range
📑PMC: 7399350; PMC: 10093564
#SR18292 #WesternBlot #WB #Troubleshooting

🧪 SDS-PAGE is a core protein analysis technique 🔬, helping students separate, identify, and study protein size and purity accurately 📊.

#SDSPAGE #ProteinAnalysis #BiochemistryLab #MolecularBiology #LifeScienceStudents #WesternBlot #LabTechniques #Biotechnology #ResearchLabs #BioResire

GeneTex is the leading manufacturer of #zebrafish #antibodies
All have been tested on zebrafish samples in a variety of applications including, but not limited to, #immunofluorescence, #immunohistochemistry & #westernblot
Find your target here: www.stratech.co.uk/genetex/zebr...

#animalmodels

Knockout (KO) Validated Antibodies | Antibodies.com Choose from 500+ knockout (KO) validated antibodies for your research, including antibodies for WB, IHC, ICC/IF, ChIP, ELISA...

We offer hundreds of #antibodies that were knock-out validated for you: www.antibodies.com/products/pri...

In the image here is a #WesternBlot analysis of extracts from wild type (WT) and Cyclin D1 knockout #HeLa cells, using Anti-Cyclin D1 Antibody (A306339): www.antibodies.com/catalog/prim...

New Year, new project? We offer thousands of #antibodies at 10 µL vials, so you can test the #antibody first before committing to a full-size vial. Check out our full range: www.antibodies.com/products/pri...
#CellBiology #MolecularBiology #Biochemistry #ImmunoFluorescence #WesternBlot

One of the best ways to validate the specificity of #antibodies is to use #knockout cells as control: If a #WesternBlot band or #ImmunoFluorescence staining appears in the wild type cells and not in the knockout cells, it means the antibody is specific to the desired #protein.

Why else? flexibility! Pick and choose your reporter. Use one antibody and conjugate it to #AlkalinePhosphatase for #ELISA, #horseradishperoxidase for #WesternBlot or a #fluorophore for #Immunofluorescence. Check out our range of biotinylated secondary antibodies:
www.antibodies.com/products/sec...

Why researchers choose Bovine Serum #Albumin (#BSA) from Proliant Health & Biologicals?

Batch-to-batch uniformity min contaminants no detectable IgG or protease, suitable for a range of assays & blocking protocols in #ELISA #Westernblot & #flowcytometry 

🔗https://tinyurl.com/yftm27tt

#diagnostics

Proteintech offers tiered discounts up to €625 on antibodies, ELISA kits, and proteins in 2025 with promo code EUSPENDSAVE.

Spend more, save up to €625! 🧪
Our UK/EU promo ends 30 November. Stock up on antibodies, assays and nanobodies while it lasts.

Use code EUSPENDSAVE at checkout (T&Cs apply)
Find out more: http://bit.ly/47gmwxs

#Antibodies #WesternBlot #Proteintech

Western Blot Protocol Step by step protocol for Western Blots, including planning advice, analysing results, recipes for solutions and troubleshooting.

Western Blot Protocol #westernblot #westernblotting
➡️ hellobio.com/western-blot...

6 Essential Protocols for Life Science Researchers Solid, reliable protocols are the key to turning tricky experiments into meaningful results. From Western blots to ELISAs, we’ve rounded up six essential step-by-step guides that scientists around the...

Looking for help with your Western blot? Need support with your ELISA assay? 🤔

Reliable protocols can turn tricky experiments into meaningful results & that's why we’ve put together 6 essential protocols for scientists: ow.ly/vWvT50X6XCb

#westernblot #elisaassay #amyloid #amyloidbeta #protocols

On the left - western blot of B16F10 cells wt and KO for CDK8. Our in house produced antibodies give a lot of unspecific bands. On the right same probes with antibodies preincubated with fixed CDK8 KO cells - there is a specific band and faint unspecific bands, which can be probably eliminated with increase of amount of KO cells.

Neat trick if you polycolonal ab's suck. Incubate them with fixed cells with a KO of your protein of interest, then spin. Protocol here: www.med.upenn.edu/markslab/ass...
I was amazed how well it worked on first try (I'm sure that I can completely eliminate unspecific bands)
#WesternBlot #cellsky

1st western blot vs. 128th western blot 🧪
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#science #westernblot #predoc #labscientist

Want to standardize your workflows and protocols?
Our lyophilized exosome standards (from CD63-EGFP HEK or human thrombocytes) support #FNTA, #FlowCytometry, #ELISA & #WesternBlot.
Reliable. Consistent. Stable.
🔗 particle-metrix.com
#Standardization #NTA @particle-metrix.bsky.social

Frustrated by inconsistent Westerns?

Our new free online course covers everything from sample prep to result analysis.

Start learning at learn.ptglab.com 🧪🥼
#proteintech #westernblot #proteintechlearningportal

Free Western blotting online course now available on the Proteintech Learning Portal.

Enroll here: learn.ptglab.com/westernblot?...

#WesternBlot #FreeCourse #ProteinDetection #PhDTraining #LearningPortal

🚨 Excited to exhibit at #BIO2025 in Boston! Visit us at Booth #447 to see how our G-Blot system runs 96 samples with your standard reagents & protocols—faster, easier, & cost-effective. DM us or email cameron@blottinginnovations.com to connect! #WesternBlot #HighThroughput

PVDF vs Nitrocellulose? How to Choose a Blot Membrane 🤔
If you're optimizing your blots, this guide from Fisher Scientific breaks down when and why membrane matters:

📌 Also includes practical tips for handling and troubleshooting.

#WesternBlot #LifeScienceTips

Findings of Research Misconduct Findings of research misconduct have been made against Liping Zhang, Ph.D. (Respondent), former Assistant Professor in the Department of Medicine, Section of Nephrology, Baylor College of Medicine. Re...

#NIH Office of Research Integrity bars former Baylor nephrologist from receiving federal funds for 2 years after finding that she engaged in research misconduct.

#fraud #sleuth #westernblot #Medsky #nephrology #research

Difference between small and large pore-size WB membranes Detailed breakdown of the difference between small and large pore-size WB membranes

𝗗𝗼 𝘆𝗼𝘂 𝗸𝗻𝗼𝘄 𝘁𝗵𝗲 𝗱𝗶𝗳𝗳𝗲𝗿𝗲𝗻𝗰𝗲 𝗯𝗲𝘁𝘄𝗲𝗲𝗻 𝘀𝗺𝗮𝗹𝗹 𝗮𝗻𝗱 𝗹𝗮𝗿𝗴𝗲 𝗽𝗼𝗿𝗲-𝘀𝗶𝘇𝗲 𝗺𝗲𝗺𝗯𝗿𝗮𝗻𝗲𝘀? Grab the explanation below 👇 and let's ace those experiments together!
#westernblot #molecularbiology #labwork

A 96-Well Polyacrylamide Gel for Electrophoresis and Western Blotting Western blotting is a stalwart technique for analyzing specific proteins and their post-translational modifications. However, it remains challenging to accommodate more than ∼10 samples per experiment...

🚀 Exciting news! Our research on a 96-Well Western blot is now published in #ACS! 🎉

We introduce a high-throughput, efficient, and reproducible #WesternBlot system that works within standard workflows—scaling up protein analysis like never before! 🔬

pubs.acs.org/doi/10.1021/...

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social

Cover der neuen Laborjournal-Ausgabe 1-2/2025 mit KI-generiertem Titelbild von James Bond im Laborkittel, der dem Betrachter mit einem Martini zuprostet. Darunter der Titel "Sicherheitsprüfungen – Spione im Labor". Ganz unten in drei Kästen nebeneinander Ankündigungen dreier weiterer Artikelthemen des abgebildeten Hefts.

Unser Heft 1-2/2025 ist raus!

Titelthema: Wissenschaftsspionage

Außerdem:
- #KI in der #Genomik;
- #Wisskomm: Comic-Projekt
- #Machtmissbrauch
- Entwicklungsbiologie;
- #CRISPR-Cas;
- #Geldokumentation;
- #WesternBlot
- u.v.a.m.

Das ganze Heft online: www.laborjournal.de/rubric/aktue...

Foto: Drei Finger in einem Laborhandschuh halten ein Glasfläschchen mit einer orange fluoreszierenden Flüssigkeit seitlich ins Bild. Rechts daneben Überschrift "In-Gel-Fluoreszenz ersetzt Western Blot" des im Post verlinkten Artikels, gefolgt von dessen Vorspann.

Viele Fluoreszenzproteine bleiben auch im SDS-Gel intakt, sodass man sie direkt im Gel detektieren kann. Auf welche Weise solche In-Gel-#Fluoreszenz die aufwendige #Proteindetektion via mühsamen #WesternBlot ersetzen kann, erklärt uns Andrea Pitzschke: www.laborjournal.de/editorials/3...