Top: Inhibition of mTOR signaling rescues cilia formation in Wnt activated cells. RPE1 cells stably expressing mCherry-GFP-LC3 were treated with Co-CM and Wnt3a-CM and fixed 16 h after serum starvation for direct fluorescence analysis of mCherry and GFP signals. Representative images show GFP+mCherry+ foci (yellow dots, autophagosomes). Bottom: Model of how Wnt signaling affects ciliogenesis. Basal Wnt signaling promotes cilia formation, whereas Wnt hyperactivation prior to ciliogenesis delays this process by increasing mTORC1 activity and impairing the removal of OFD1 from centriolar satellites. M, mother centriole; D, daughter centriole.

The #PrimaryCilium regulates several signaling pathways, but what role do these pathways play in #cilium formation? This study shows that modulating Wnt & mTOR signaling affects #ciliogenesis in human retinal epithelial cells @plosbiology.org 🧪 plos.io/45Yg5gB

Top: Inhibition of mTOR signaling rescues cilia formation in Wnt activated cells. RPE1 cells stably expressing mCherry-GFP-LC3 were treated with Co-CM and Wnt3a-CM and fixed 16 h after serum starvation for direct fluorescence analysis of mCherry and GFP signals. Representative images show GFP+mCherry+ foci (yellow dots, autophagosomes). Bottom: Model of how Wnt signaling affects ciliogenesis. Basal Wnt signaling promotes cilia formation, whereas Wnt hyperactivation prior to ciliogenesis delays this process by increasing mTORC1 activity and impairing the removal of OFD1 from centriolar satellites. M, mother centriole; D, daughter centriole.

The #PrimaryCilium regulates several signaling pathways, but what role do these pathways play in #cilium formation? This study shows that modulating Wnt & mTOR signaling affects #ciliogenesis in human retinal epithelial cells @plosbiology.org 🧪 plos.io/45Yg5gB

Top: Inhibition of mTOR signaling rescues cilia formation in Wnt activated cells. RPE1 cells stably expressing mCherry-GFP-LC3 were treated with Co-CM and Wnt3a-CM and fixed 16 h after serum starvation for direct fluorescence analysis of mCherry and GFP signals. Representative images show GFP+mCherry+ foci (yellow dots, autophagosomes). Bottom: Model of how Wnt signaling affects ciliogenesis. Basal Wnt signaling promotes cilia formation, whereas Wnt hyperactivation prior to ciliogenesis delays this process by increasing mTORC1 activity and impairing the removal of OFD1 from centriolar satellites. M, mother centriole; D, daughter centriole.

The #PrimaryCilium regulates several signaling pathways, but what role do these pathways play in #cilium formation? This study shows that modulating Wnt & mTOR signaling affects #ciliogenesis in human retinal epithelial cells @plosbiology.org 🧪 plos.io/45Yg5gB

Soon to be #NewPI Yinwen Liang (Joyner lab, MSKCC) on informatics approach to find novel transcriptional regulators of #ciliogenesis- SP5 and SP8 specificity protein TFs drive expression of core ciliary machinery in primary & motile cilia! www.biorxiv.org/content/10.1... #UKCilia2025 /3

FUT8-mediated core #fucosylation stabilizes TMEM67 to promote #ciliogenesis, say Difei Wang, Huijie Zhao, Jun Zhou and colleagues (Shandong Normal University): rupress.org/jcb/article/...

#Cilia #Disease

In @jcb.org, Wang et al. show that FUT8-mediated core #fucosylation stabilizes TMEM67 to promote #ciliogenesis and that FUT8 deficiency in mice causes ciliary defects in multiple organs. rupress.org/jcb/article/...

#Cilia #Disease

Wang et al. show that FUT8-mediated core #fucosylation stabilizes TMEM67 to promote #ciliogenesis and that FUT8 deficiency in mice causes ciliary defects in multiple organs. rupress.org/jcb/article/...

#Cilia #Disease

Deeply saddened by the news that David Stephens died last night.
David was an outstanding cell biologist based at Bristol whose work spanned #MembraneTraffic #cytoskeleton #ciliogenesis + more. He was “one of the good guys”. Kind, supportive, generous with his time, and rigorous about his science. 🧪

Early-stage defects of Lung morphogenesis in Inturned (a #Ciliogenesis protein) mutant🐭

Delayed budding of Bronchi primordia at e10.5
⏬Airway #BranchingMorphogenesis until e13.5
⏬epithelial/mesenchymal Ciliogenesis
⏬HedgeHog signals

@devbiol.bsky.social 2024
www.sciencedirect.com/science/arti...

PATJ, a Crumbs polarity complex protein,⏫epithelial cell #Lumenogenesis #Ciliogenesis

PATJ exhibits junctional+ciliary localizations, &⏬HDAC7, a ciliary protein

HDAC7 KD rescues morphogenetic defects of PATJ crispant MDCK cells

#CellMolLifeSci 2023
link.springer.com/article/10.1...