New preprint out!
In this study, we introduce NanoFLex, a strategy that combines #HaloTag variants with #nanobodies -based immunolabeling to enable rapid, OneStep-IF #lifetime #multiplexing. Possible due to SmartSecondaries fused to #HaloTag from @nanotag.bsky.social: www.biorxiv.org/content/10.6...

Designing proteins for targeted cell activation Prof. Thomas Schlichthärle spricht über seine Arbeit als Professor für AI-guided Protein Design.

Get to know Thomas Schlichthärle, our newly appointed professor of #AI-guided #ProteinDesign, in the latest NewIn episode. He wants to use custom-designed #proteins to advance the development of new #medicines: go.tum.de/484027

#HightechAgendaBayern

▶️ youtu.be/G-pt28HJpA8

📹& 📷ProLehre

Super cool work !

Kinetic Insights Into Carbon Nanotube-Based Neurotransmitter Sensors by Single Molecule Experiments Single-walled carbon nanotubes (SWCNTs) are powerful building blocks for near-infrared (NIR) fluorescent biosensors. They can be chemically tailored to detect specific biomolecules. Their performance ...

Congrats to Juliana! She measures in ACS Nano the rate constants of nanosensors on the single molecule level. She found two kinetic pathways and one can use this knowledge for Kinetically Improved Sensor Selectivity (KISS). @solvationsci.bsky.social @ruhr-uni-bochum.de
tinyurl.com/738ypxf9

Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells Single-molecule localization microscopy (SMLM) has redefined optical imaging by enabling imaging beyond the diffraction limit, allowing for nanoscale investigation into cellular architecture and molec...

Excited that our work on Single-objective lattice light sheet microscopy with microfluidics for single-molecule super-resolution imaging of mammalian cells is now published in ACS Photonics!

doi.org/10.1021/acsp...

@pubs.acs.org #soTILT3D #soLLS #microscopy #nanoscale #imaging

✨ Blinking #nanobodies that work for single-molecule localization 🔬
Our new preprint shows that the self-blinking dye JF635b restores robust, buffer-free blinking in #nanobodies, enabling reliable #dSTORM, #MINFLUX, and more, without chemical-switching buffers. Opening new possibilities for #ExM!

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Direct STORM relies on robust fluorophore blinking; observations suggest that AlexaFluor647 underperforms when coupled to nanobodies, thereby undermining the advantages of nanobodies. We show that the...

Self-blinking dye restores efficient use of nanobodies in single-molecule localization microscopy. Link ot he @biorxivpreprint.bsky.social is here: www.biorxiv.org/content/10.6...

A cool thing was the FRET-based diffusion analysis that allowed a separate analysis of MET receptor monomers and dimers👇🏻

Super-resolved Imaging of Molecular Interactions Using FRET-SOFI Super-resolution fluorescence microscopy is a powerful method to image molecular processes that occur beyond the diffraction limit of light. Förster resonance energy transfer (FRET) is widely used to ...

Excited to share our latest work! We introduce FRET-SOFI, a new method for super-resolved imaging of molecular interactions. By combining SOFI with FRET, we achieve subdiffraction resolution in live cells using standard wide-field microscopy.
pubs.acs.org/doi/10.1021/...

This #Halloween 🦙🧛‍♂️ Alpacula brewed up #SMLM treat!
No more horror stories of no blinking & black holes ⚫️🔬Our single-domain anti-BOOdies 👻 #FluoTags and #SmartSecondaries now glow with self-blinking JF635b✨, delivering frightfully precise and simple #dSTORM imaging. 👉 bit.ly/4ffIkdB

We present a versatile microfluidics platform that makes multiplexed super-resolution imaging more accessible, reproducible, and high-throughput.

👉 BioRxiv: www.biorxiv.org/content/10.1...

#SuperResolution #DNApaint #Microfluidics #SMLM #OpenScience #multiplexing

Spatial mapping of cell-surface protein glycosylation at molecular resolution Cell-surface protein glycosylation is a fundamental post-translational modification that plays a crucial role in membrane protein function and cellular behavior. However, elucidating the molecular spa...

NEW PREPRINT: Spatial mapping of cell-surface protein glycosylation at molecular resolution 🍭🍬🔬
For the first time, we can analyze the spatial architecture of the cell-surface glycoproteome at single-protein and single-sugar resolution. #glycotime

www.biorxiv.org/content/10.1...

Fiery kickoff for the 30th Single Molecule Workshop!
I’m super excited to see so many great scientists and big names in the field gathered here in Berlin.
Let’s go #WS30 🚀

#WS30 #SingleMolecule #Spectroscopy #Microscopy

Soo on stage presenting self-labeling enzymes with weak-affinity fluorophore labels for (1) high-density live SMLM using DeepSTORM (doi.org/10.1016/j.bp...) and (2) molecular counting ... great collaboration with @kjohnsson.bsky.social @juliankompa.bsky.social 🙏 #PQ30 @picoquant.bsky.social

Absolutely amazing !

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

Previous preprint is now published in RSC Chemical Biology.
If you are interested in plasma membrane labeling, see the paper! 👇
pubs.rsc.org/en/content/a...

Happy to contribute to this new work from @micoxscopy.bsky.social et al, helping apply SSQUASH to analyse the shape of spectrin rings along axons. Data analysis is the next frontier in SMLM, and I'm always up for chatting, sharing data and see how we can push things further!

🚨
New Preprint!
Ex-dSTORM resolves fine details of the molecular architecture of CCPs, the 8-nm periodicity of microtubules, and the docking site of synaptic vesicles at the presynapse of hippocampal neurons.

▶️ www.biorxiv.org/content/10.1...

🚨 New preprint on bioRxiv! 🚨
Smart microscopy🔬 is reshaping life sciences—but progress is slowed by fragmentation.
@lhinderling.bsky.social & the Smart Microsopy Working Group maps the field & proposes a path toward an open, interoperable future.
🧵👇
doi.org/10.1101/2025...

The common discussion topic for SMLMS2025

Proud to see this preprint out!

First time demonstrated Confocal MIET DNA-PAINT and has been a challenging but rewarding journey, and I’m excited about the possibilities it opens for 3D multiplexed nanoscopy in cells.

Looking forward to advancing nanoscale imaging and molecular mapping at LMU Munich, and to many more exciting synergies ahead!

Excited to share — our project from @labtinnefeld.bsky.social with Heinrich Grabmayr from @jungmannlab.bsky.social has been selected for the CeNS Investment Fund from @cens-lmu.bsky.social !

www.cens.lmu.de/en/research/...

💥 We have 3 positions open in my lab in Basel, Switzerland🇨🇭:
#nanopores #smFRET #biomolecular #dynamics #singleMolecules

We’re looking for talented & ambitious new colleagues enthusiastic about biomolecular dynamics & single-molecule tech.

Please share broadly, thank you!🤝

schmid.chemie.unibas.ch

Great lab and great PI to start with ...!

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

🔬🧬 New in @natcomms.nature.com‬: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess 

www.nature.com/articles/s41...

@ellenberglab.bsky.social @jungmannlab.bsky.social
#Genomics #SuperResolution

A "Small" update on studying the coating of single DNA nanostructures with a fluorescence lifetime sensor! 🧬🔬 Was a fun project at the interface of DNA nanotech and single molecule photophysics working in a great team! 🤓 🙌 @ameliehj.bsky.social

onlinelibrary.wiley.com/doi/10.1002/...

It's here! ✨
The #ExSRRF protocol paper is out 😍. Full details for merging Expansion Microscopy with our eSRRF analysis. From cells to tissue. A partnership with @puellesv.bsky.social's lab. Lead by Dominik Kylies and @hannah-superres.bsky.social + colleagues. #ExM #eSRRF
➡️ Open link: rdcu.be/euvEw

🎉 Major achievement for #LMU in the Excellence Strategy: All seven proposed Clusters of Excellence have successfully passed the review process and will be funded for seven years as of 2026. An outstanding result! 🎉 #ExcellenceCluster #LMUMunich #research Read more here: www.lmu.de/en/newsroom/...