For my #proteomicsky & #massspecsky nerds, we used a S-Trap from @protifi.bsky.social to prepare samples for LCMS. We also used DSSO as our crosslinker & analyzed it with a FAIMS Pro Duo equipped Orbitrap Ascend from @thermofishersci.bsky.social for both sHCD MS2 & CID-HCD MS2-MS3 methods.

As I linked to before, these manuscripts can be found @biorxivpreprint.bsky.social. And just so we get the right audience… #medsky #sciencesky #cryoEM #proteimics #systemsbiology #crosslinking #structuralbiology #epigenetics #transcription #chromatinbiology #proteomicsky #massspecsky

#chemsky #massspecsky #massspec #ptoteomicsky

#secmet
#massspecsky
#ethnobotany
#chemsky

-"You can detect small molecules in people to see what kind of trouble they're up to"

-"It meets my self-fulfillment needs (Pyramid image from lecture 1 slide 9 and Maslows hierarchy of needs)."

#secmet
#massspecsky
#chemsky

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Start Page: /home/software/Skyline/events/2025 UW Course

Pleased to announce the summer's Skyline Targeted Quantitative Mass Spectrometry Course - the week of July 7-11, 2025 at the University of Washington in Seattle. Details and registration information can be found here: skyline.ms/project/home...
#massspecsky #proteomicssky #proteomics #skyline

#MassSpecSky #ProteomicSky #MicroSky

Machine Learning-based Classification for the Prioritization of Potentially Hazardous Chemicals with Structural Alerts in Nontarget Screening Nontarget screening (NTS) with liquid chromatography high-resolution mass spectrometry (LC-HRMS) is commonly used to detect unknown organic micropollutants in the environment. One of the main challeng...

We’ve just published a new approach to prioritise potentially hazardous chemicals in non-target screening for environmental contaminants #HRMS #NTS #NTA #MassSpec #MassSpecSky #environment

pubs.acs.org/doi/10.1021/...

Start Page: /home/software/Skyline/events/2025 User Group Meeting at ASMS/abstract-form

Attention Skyline Users? Going to ASMS and have an interesting application of Skyline or Panorama? March 14th is the abstract deadline for the Skyline User Group Meeting. skyline.ms/project/home...
#proteomicsky #massspecsky #massspec

Start Page: /home/software/Skyline/events/2025 UW Course

2025 Quantitative Proteomics Course in Seattle WA. Space is limited, register now! This is the 11th year of offering this weeklong course.
skyline.ms/project/home...
#proteomicsky #massspecsky #massspec

QCxMS2 – a program for the calculation of electron ionization mass spectra via automated reaction network discovery We present a new fully-automated computational workflow for the calculation of electron ionization mass spectra by automated reaction network discovery, transition state theory and Monte-Carlo simulat...

Our paper @grimmelab.bsky.social on QCxMS2 for the calculation of EI-MS was just accepted by @pccp.rsc.org Check out the final article: pubs.rsc.org/en/content/a...
#CompChemSky #MassSpecSky

Using the Hunt lab peptide sequencing tutorial in our upper level-UG Bioanalytical Chemistry course and it’s great. Just had fun doing the examples by hand — now it’s the students turn! #massspecsky

#MassSpecSky #EpiSci

#MassSpecSky please save me i am floating in an ocean of ammonium bicarbonate buffer

A table from an Agilent TOF showing the Resolution, Primary Residuals, and Corrected Residuals columns. The Resolution values span 6,186 to 19,958.

Ah, nothing quite like a PM visit and a fresh system tune to put your TOF-MS resolution back on track. #chemsky #RealTimeChem (is #MassSpecSky a thing?)

Within #metabolomics, reliable MS2 annotation is critical, but there's incredible variability in fragmentation depending on MS voltage. What a cool new paper out today that looks forwards to more concerted approaches using 20 eV! #massspecsky
pubs-acs-org.ezaccess.libraries.psu.edu/doi/10.1021/...

Looking forward to this!

#MassSpecSky
#ProteomicsSky-Hot

Amplifying for #TeamMassSpec #MassSpecSky, applications are now open for the Darlene Soloman award to attend iSLS and the Agilent Global Solutions Development Centre in Singpore next year. Great for anyone hoping to break into #lipidomics femalesinms.com/darlene-solo...

Hi #MassSpecSky! On behalf of ANZSMS, the IMSF and the #IMSC2024 local organising committee we warmly invite you to join us next year in Melbourne from August 17-23. Please visit our website www.imsc2024melbourne.com and sign up to our mailing list to stay updated. #TeamMassSpec