Very much looking forward to the @led3hub.bsky.social Lecture by Christina Woo (@thewoolab.bsky.social)! I am sure it will be a great one.

If you are interested in #ChemicalProteomics and/or targeted protein degradation #TPD, you will not want to miss it.

#ChemSky #ChemBio #DrugDiscovery #degrader

We are looking forward to the LED3 Lecture of Christina Woo (@thewoolab.bsky.social) on February 26th. She will talk about "Rewriting Protein Modifications".

If you are interested, make sure to come by!

www.universiteitleiden.nl/en/science/l...

#ChemBio #ChemSky #ChemicalProteomics #TPD

An interactive resource mapping the proteome and reactive cysteine landscape across the NCI-60 reveals cell and tissue-specific profiles Montaño et al. generate an integrated proteomic and cysteine reactivity resource for the NCI-60 cancer cell line panel, offering quantitative data on over 12,000 proteins and 36,000 cysteines to enabl...

Which cysteines are hyper-reactive in specific cancer types and tissues?

The lab of @balynzaro.bsky.social addresses this question by measuring cysteine reactivity across the NCI-60 panel that includes 9 different cancer types.(1/2)
www.cell.com/cell-chemica...
#ChemBio #ChemSky #ChemicalProteomics

Excited to be at the meeting of the Institute for Chemical Neuroscience today. Looking forward to a great day of learning about #neuroscience on the clinical and molecular level as well as to discussing, how chemical tools can drive the field forward
i-cns.nl/en
#ChemSky #ChemBio #ChemicalProteomics

#Chemistry #ChemBio #ChemicalProteomics #ChemPro #ProteoProbes #DrugDiscovery #Glycotime #Peptides #Materials #Synthesis #Undruggable #CovalentInhibitors
(3/3)

Chemical proteomic profiling reveals prostaglandin termination enzyme PTGR2 as a key molecular target of natural coumarin fraxetin Natural coumarins represent a diverse group of secondary metabolites with a wide range of biological activities. However, their specific molecular targets have remained largely unexplored. Employing chemical proteomics, a comprehensive analysis of the protein targets of the natural coumarin fraxetin has been

Interesting talk by Weining Zhao at #ISCPDD. He talked about the use of #ChemicalProteomics to study the target proteins of Fraxetin. They identified PTGR2 as a relevant target in human cells.

pubs.rsc.org/en/cont...
#ABPP #AfBPP #ChemSky #ChemBio #ProteoProbes #ChemPro

FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis - Nature Chemistry A naturally occurring stand-alone and intermolecular Diels–Alderase, MaDA, has been identified from Morus alba cell cultures. MaDA is a FAD-dependent enzyme, which catalyses the intermolecular [4+2] c...

Great talk by Xiaoguang Lei at #ISCPDD. He talked about the use photoaffinity probes based on biosynthetic intermediates to fish for the relevant enzymes to identify e.g. an intermolecular Diels-Alderase.
www.nature.com/articles/s41...
#ChemSky #ChemBio #ChemicalProteomics #ChemPro #ProteoProbes

Great to see our Nature Chemistry paper out in its final published and printed form.

If you want to check it out, you can find it here: www.nature.com/articles/s41...

#ChemSky #ChemBio #ChemicalProteomics #Chemoproteomics #ProteoProbes #ChemPro

Ketoboronate as a Minimal Covalent-Reversible Tag for Targeted Lysosomal Degradation of Extracellular and Membrane Proteins Extracellular and membrane-associated proteins play essential roles in nearly all our body’s biochemical processes and are implicated in cancer, autoimmune disorders, and neurodegenerative diseases. Consequently, a selective and universally applicable technique for the degradation of these proteins in disease-relevant conditions could significantly improve human health prospects. Lysosome-targeting chimeras (LYTACs) are bifunctional degraders comprising of an antibody conjugated with a cell–surface receptor ligand that enables cargo lysosome shuttling and degradation. Herein, we demonstrate that conjugation of antibodies with a small ketoboronate-based, lysine-reactive, covalent-reversible uptake tag (KB) enables the internalization of plasma membrane and extracellular proteins, directing them to lysosomal degradation via receptor-mediated endocytosis. Chemoproteomic target deconvolution revealed that reversible modification of lysine residues on the transferrin receptor protein 1 (TFRC) and HLA class I histocompatibility antigen A, B and C (HLA-ABC) enabled efficient uptake and lysosomal targeting through both clathrin-dependent and -independent mechanisms. KB-antibody conjugates (KB-TACs) efficiently degrade the epidermal growth factor receptor (EGFR), vascular endothelial growth factor A (VEGFA) and epidermal growth factor receptor 2 (HER2) in cancer cells. Furthermore, we showed KB-TTZ degrades HER2 in vivo in BT-474 tumor xenografts, with a significant reduction in tumor volumes compared to TTZ and vehicle treatments. Altogether, the KB tag represents a versatile, minimal-size chemical unit to functionalize therapeutic antibodies for targeted protein degradation through dual receptor-mediated endocytosis and lysosomal delivery.

Exciting study by the group of Alexander Adibekian in JACS. They used antibodies modified with reversible, lysine-directed ketoboronates to develop KB-TACs as lysosomal degraders of membrane and extracellular proteins.

doi.org/10.1021/jacs...
#ChemSky #ChemBio #ChemicalProteomics #DrugDiscovery

Amazing lecture by Zhengqiu Li at #ISCPDD. He presented his group’s exciting research into the recruitment of new E3 ligases for targeted protein degradation using diverse new electrophiles including of NEDD4 via an azirine.
pubs.acs.org/doi/full/10....
#TPD #ChemSky #ChemBio #ChemicalProteomics

Geat talk by Yu Rao at #ISCPDD. He talked about his group’s work on PROTACs and the use of degradation-based protein profiling (DBPP) to characterize target engagement.
advanced.onlinelibrary.wiley.com/doi/10.1002/...
#TPD #ChemSky #ChemBio #DrugDiscovery #ChemicalProteomics #ChemPro #ProteoProbes

They also compared their identified ligand binding sites to those predicted by Boltz-2. They find that Boltz-2 predicts orthosteric binding well, but tends to also localize non-orthosteric binders to the orthosteric binding site. (2/2)
#ChemSky #ChemBio #ChemicalProteomics #ChemPro #ProteoProbes

A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands - Nature Methods The peptide-centric local stability assay (PELSA) can be used to determine ligand-binding targets and binding regions on a proteome-wide scale with high sensitivity.

Insightful talk by Mingliang Ye at #ISCPDD. She talked about the use of #ChemicalProteomics for the study of target engagement in complex biological systems. Exciting insights into peptide-centric local stability assay (PELSA) and SICFA. doi.org/10.1038/s415...
#ChemSky #ChemBio #DrugDiscovery

Zhengqiu Li opens the 3rd International Symposium on Chemical Proteomics and Drug Discovery #ISCPDD in Guangzhou marking the start of two days of exciting reports on research in #ChemicalProteomics, #ChemBio, #Chemistry and #DrugDiscovery.
#ChemSky

Probing the proteome - Nature Biotechnology Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?

I envy the glycoscience community for their #glycotime hashtag. A great way to make posts visible. Anyone in #ChemicalProteomics that has a good idea, which hashtag we might use to make all of our work more visible and findable?
#ChemSky #ChemBio #DrugDiscovery #ABPP
www.nature.com/articles/s41...

A big thank you also to Stacey Paiva and her team at Nature Chemistry for their support and patience with me.

#CovalentInhibitor #DrugDiscovery #Proteomics #ChemicalProteomics #MassSpectrometry #Electrophiles #ProudPI #AcademicLife #NewPublication #Chemistry #ChemBio #ChemSky
(6/7)

This great @science.org paper by Vividion and @crick.ac.uk headed by Matt Patricelli and @juliandownward.bsky.social on covalent inhibitors of the PI3Kα-RAS interaction. Exciting project on #CovalentInhibitors and #ChemicalProteomics.
www.science.org/doi/10.1126/...
#ChemSky #ChemBio #DrugDiscovery

Profiling the proteome-wide selectivity of diverse electrophiles Targeted covalent inhibitors are powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine residues. The development of cysteine-directed covalen...

Interested in the proteome-wide selectivity of diverse electrophiles?

The proteomics data for our study on this headed by @pzanon.bsky.social are now public on @pride-ebi.bsky.social: www.ebi.ac.uk/pride/archiv...

Full story: chemrxiv.org/engage/chemr...

#ChemBio #ChemSky #ChemicalProteomics

Excited that Christina Woo (@thewoolab.bsky.social, @harvard.edu) will visit us for a @led3hub.bsky.social Lecture.

Looking forward to hearing more about her group's exciting research in the fields of #ChemBio, #ChemicalProteomics, #ChemSky and #ProteinDegradation.

www.nature.com/articles/s41...

We look forward to the LED3 Lecture by Christina Woo (@thewoolab.bsky.social) on February 26th, 2026. Her group performs exciting research in the field of #ChemBio and #ChemicalProteomics to understand small molecule activity in the proteome.

Already mark your calendars! www.universiteitleid...

Target identification of natural products in cancer with chemical proteomics and artificial intelligence approaches <p xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="first" dir="auto" id="d6020129e153">Natural products (NPs) have long been recognized for their therapeutic potential, especi...

'Target identification of natural products in cancer with chemical proteomics and artificial intelligence approaches' - a Cancer Biology & Medicine (CBM) article on #ScienceOpen:

🔗 www.scienceopen.com/document?vid...

🖇️ #CancerBiology #NaturalProducts #CancerTreatment #ChemicalProteomics

Attending #DrugDiscoveryChemistry 2025 in San Diego?🔬 Make sure to meet us at Booth #606.👋

Discover how our #ChemicalProteomics services powered by proprietary LiP-MS technology can advance your #DrugDiscovery projects!

Learn more: biognosys.com/conferences/...

@acs.org #DDC25

Next week, meet us at Drug Discovery Chemistry 2025 in San Diego, CA!🏖️🔬

👋 Booth 606
📅 April 14–17, 2025
📍 San Diego, CA

Discover how our #ChemicalProteomics platform, #TrueTarget can accelerate your #DrugDiscovery projects.

Learn more: biognosys.com/conferences/...

Lipidation of proteins has diverse essential roles in biology.

In his amazing keynote lecture at #Lunteren, Marc Baggelaar talked about his group's exciting contributions to better understand protein lipidations using #ChemicalProteomics. (1/5)
#chemsky #ChemBio

If you are looking for exciting scientific content (with a focus on #ChemBio, #ChemSky, #ABPP, #ChemicalProteomics), check out these starter packs:
short list: go.bsky.app/C4URhFa
pack 1: go.bsky.app/3SyoVNw
pack 2: go.bsky.app/9keb2TP

Verhelst Lab – Laboratory of Chemical Biology

Hello #chemicalbiology community on bluesky! Some of you may know my lab. We work on covalent chemical probes with a main focus on proteases. #abpp #chemicalproteomics You can visit www.verhelstlab.net or check our papers on pubmed.

Thank you, @keribackus.bsky.social, for making a specific Starter Pack for #ChemicalProteomics #Chemoproteomics. Many exciting accounts of the field.

The 2025 Bioorganic Gordon Research Conference organized by Denise Field and @doc-jlmeier.bsky.social has an amazing lineup of speakers. Sadly, I will not be able to travel, but if you are interested in #ChemicalBiology, #BioorganicChemistry or #ChemicalProteomics, make sure to check it out. (1/4)

Very excited to see the scientific community forming over here and to have reached 500 followers. I will try to ramp up my activities again posting on literature on #Science, #Chemistry, #Chemsky, #ChemicalBiology, #Antibiotics, #CovalentInhibitors, #ChemicalProteomics and #ABPP.