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Three female researchers discuss data displayed on dual monitors in a microscopy workspace with keyboards, mouse, and electronic equipment on the desk.

Three female researchers discuss data displayed on dual monitors in a microscopy workspace with keyboards, mouse, and electronic equipment on the desk.

Two female researchers working at a computer displaying a 3D scientific visualization on a large curved monitor in a modern office.

Two female researchers working at a computer displaying a 3D scientific visualization on a large curved monitor in a modern office.

Two young researchers examining an object with tweezers in a lab setting near a sink and hygiene supplies.

Two young researchers examining an object with tweezers in a lab setting near a sink and hygiene supplies.

Group of participants in a lab setting reviewing microscopy images on computer screens and looking at printed diagrams during a practical course.

Group of participants in a lab setting reviewing microscopy images on computer screens and looking at printed diagrams during a practical course.

We are having a very hands-on week at the @embo.org #cryoFIB & #cryoET course 🔬

Our participants are learning about sample vitrification, lamella milling, FLM, lift-out, data acquisition and more!

We are learning by doing, troubleshooting, and sharing expertise across labs. 🧪

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Three young researchers—two women and one man—stand in front of a poster at a poster session, holding drinks and smiling as they chat. Other people stand in the background.

Three young researchers—two women and one man—stand in front of a poster at a poster session, holding drinks and smiling as they chat. Other people stand in the background.

Female researcher pointing at a scientific poster on a borad while two others observe in an indoor setting.

Female researcher pointing at a scientific poster on a borad while two others observe in an indoor setting.

Female researcher pointing at a scientific poster on a borad while two others observe in an indoor setting.

Female researcher pointing at a scientific poster on a borad while two others observe in an indoor setting.

Two female researchers at the poster session are standing in front of a poster board, holding drinks and chatting, whilst other people stand in the background.

Two female researchers at the poster session are standing in front of a poster board, holding drinks and chatting, whilst other people stand in the background.

Participants shared their work and ideas in our poster session at the @embo.org Practical Course on “In-situ structural biology by Cryo-FIB and Cryo-ET” at our institute! It was a great chance to connect, discuss, and get new perspectives on #cryoFIB and #cryoET. 🌍

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Video

How I picture the #CryoET data portal. We must feed the beast!

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5/ But one key component was still missing: the high-potential acceptor 🤔🔍 Earlier studies proposed that BCRII is membrane-attached and transfers electrons to menaquinone Using in situ #cryoET, we instead found BCRII dispersed throughout the cytoplasm, pointing to a soluble high-potential acceptor

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1/ Excited to share our preprint! 🥳

Degradation of aromatic compounds, including BTEX pollutants, requires highly endergonic aromatic ring reduction. Using #cryoEM and in situ #cryoET, we show how BCRII couples electron bifurcation modules in one giant redox machine

www.biorxiv.org/content/10.6...

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Figure 4 from the preprint

Figure 4 from the preprint

We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭

www.biorxiv.org/content/10.6...

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Male bald speaker in blue shirt giving a presentation on early days of tomographic imaging in the 1980s, featuring Walter Hoppe and technical diagrams.

Male bald speaker in blue shirt giving a presentation on early days of tomographic imaging in the 1980s, featuring Walter Hoppe and technical diagrams.

Audience seated in a room attentively listening to a presentation, some holding notebooks or tablets.

Audience seated in a room attentively listening to a presentation, some holding notebooks or tablets.

Audience seated in a room attentively listening to a presentation, some holding notebooks or tablets.

Audience seated in a room attentively listening to a presentation, some holding notebooks or tablets.

Female researcher presenting colorful microscopic images at Max Planck Institute for Biophysics during the EMBO Practical Course on “In-situ structural biology by Cryo-FIB and Cryo-ET”.

Female researcher presenting colorful microscopic images at Max Planck Institute for Biophysics during the EMBO Practical Course on “In-situ structural biology by Cryo-FIB and Cryo-ET”.

We’ve kicked off our @embo.org Practical Course on #cryoFIB & #cryoET!

Participants from around the world are joining us this week for hands-on training, from lamella milling to tomogram reconstruction 🔬

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Now THIS one was quite the journey. I'm beyond happy to see this work out in the world 🥹

Huge congrats to @computingcaitie.bsky.social and also to @hugovdhoek.bsky.social and everyone else involved ♥️

with @centriolelab.bsky.social @stearnslab.bsky.social @cellarchlab.com

#Cilia #CryoET #TeamTomo

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It’s done!! Molecular architecture of the ciliary base in mammalian multiciliated cells! A study 13 years in the making 👴, thanks to collab with @stearnslab.bsky.social + @centriolelab.bsky.social & visionary work by @computingcaitie.bsky.social, who integrated native #cryoET with #XLMS & #UExM 🧪🧶🧬🔬

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#cilia #cryoet #proteomics

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Attention NY Area #cryoem #cryoet enthusiasts - The 6th Annual NY Area Cryo-EM meeting is May 27th - Features a workshop/discussion format where new results and techniques will be discussed - We are sponsoring and attending the meeting - zurl.co/ZozvM

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3D molecular structure of an AMPA receptor showing its complex assembly, with distinct coloured subunits.

3D molecular structure of an AMPA receptor showing its complex assembly, with distinct coloured subunits.

Explore how AMPA receptors drive synaptic signalling as a #postdoc in @igreger.bsky.social’s group, combining advanced structural biology (including #cryoEM & #cryoET) with imaging and electrophysiology.
More details: www.nature.com/naturecareer...
Apply by 19 APR
#PostdocJobs #ScienceJobs

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9) This was a fantastic team effort led by Suzanne Enos with a fun and insightful collaboration between the Herzik lab at @ucsandiego.bsky.social and Grotjahn lab at @scripps.edu. #cryoET #cryoEM #HerzikLab @sptlabtech.bsky.social @structurabio.bsky.social @thermofishersci.bsky.social

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4) We teamed up with @nanigrotjahn.bsky.social and @hamid13r.bsky.social to do a through examination of SurfACT with four known proteins that suffer from PO. Combining #SPA and #cryoET paired with #STA, we show that SurfACT overcomes PO for these proteins and provides insights into this problem.

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#cryoET #TeamTomo

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Stoic 🦾 from our shared student @daniil-litvinov.bsky.social predicts protein complex stoichiometry. A fun collab with @ninjani.bsky.social @torstenschwede.bsky.social - this #AI adventure beyond our core #CryoET methods was made possible by the @biozentrum.unibas.ch PhD Fellowship Program! 🧪 🧶🧬

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The new Leopard-EM software package for 2D template matching has been written with extensibility at the forefront #InSituStructuralBiology #CryoEM #CryoET doi.org/10.1107/S205...

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You want to work on carbon concentration mechanisms in algae 🫑? Go work with my (soon-to-be-former) colleague
@manondemulder.bsky.social in Vienna @gmivienna.bsky.social !
Cryo-ET 🔬 and funky bugs 🦠

#CryoET #CryoEM

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Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Still of a video installation by Isabell Bullerschen showing segmentation of a tomogram depicting T. Kivui bacteria.

Stunning video installation by Isabell Bullerschen featuring our T. kivui #CryoET data 🧡

On view until April 19th at the HEK in #Basel!

hek.ch/en/program/e...

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Simplify your plunge freezing workflow. 📉Obtain the consistent vitrification quality required for high-end structural techniques like Single Particle Analysis & Cryo-ET. Learn how with our GraVitri Plunge Cooler Series: zurl.co/p1QLV #SPA #CRYOET #MICROED #CRYOEM

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Attention NY Area #cryoem #cryoet enthusiasts - The 6th Annual NY Area Cryo-EM meeting is May 27th - Features a workshop/discussion format where new results and techniques will be discussed - We are sponsoring and attending the meeting - zurl.co/ALfj4

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It‘s nice to be back to the world of #cryoET

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Applications for the 2026 #CryoET Data Processing Workshop are due one month from today!
Learn more about this hands-on workshop and apply by March 20: myumi.ch/lsi-cryo-workshop-2026

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Pulsed #lasers integrated with #cryoEM: @fitzpatricklab.bsky.social postdoc Daniel Du aims to boost contrast in #cryoET zuckermaninstitute.columbia.edu/making-world-s-best-microscopes-biology-better
zuckermaninstitute.columbia.edu/science-life-daniel-du
elifesciences.org/reviewed-preprints/109793v1

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Preview
Postdoc Research Group Schur The Schur lab at the Institute of Science and Technology Austria (ISTA) has an open postdoc position for a highly motivated candidate to be part of our ERC-funded project ActinID. Our Group ...

Open Postdoc position in our lab!
#actin #Huntingtin #cryoET #cellbio
If you enjoy working at the interface of cell biology, structural biology, and quantitative imaging, this could be an excellent fit.

ista.ac.at/en/job/postd...

Please share and feel free to reach out if there are questions!

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Very cool environmental #CryoET. This is an exciting frontier for #TeamTomo 🧪 🌍

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Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience

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It's crazy hat party time! 🤠🥳

#Chlamy #CryoET #TeamTomo

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...and THIS is how you write a #CryoET methods section 😉

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ribosomologists take note 👇
#CryoEM #CryoET #teamtomo

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