Sex Right Ventricle Endothelium

Female (vs Male) Fisher CDF rats are resistant to Right ventricle Wall maladaptation & Microvascular rarefaction by 2 weeks post pulmonary artery banding

E2-Sphk1 pro-angiogenic cascade

#AJPHeartCirc 2024
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My continued biomedical education

Pannexins in the vasculature

A comprehensive review from Cryo-EM structure, mechanosensing & ATP-releasing to vascular diseases Atherosclerosis AAA Hypertension Edema Thrombosis

@brantisakson.bsky.social #AJPHeartCirc 2025
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#MedialArterialCalcification

Rapamycin (5 mg/kg, 3x/wk)⏫lifespan (T50 35➡️61 d) & ⏬arterial mineral density, not calcified volume, in Mgp-/-🐭

↔️Runx2
↔️Autophagy
⏫SMC contractile phenotype

Independent of mTORC2

@sthilaire.bsky.social #AJPHeartCirc 2025
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#Pericyte #ThoracicAorticAneurysm

Pericyte contractile phenotype (Not in a good sense)⏫ FGF2⏬in 👤 #ThoracicAorticAneurysm Adventitia

FGF2▶️👤Aortic Adventitial #Pericyte ⏫pro-angiogenic & migratory ⏬contractile

#AortaEd
Julie Phillippi lab #AJPHeartCirc 2025
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Use #FourCoreGenotypes 🐭 to dissect the contributions of Sex hormones (▶️matricellular components) vs Sex chromosome (▶️mechanics) to #AorticStiffness

12~15 wk🐭

Uniaxial #ElasticModulus
Intact aorta: XXF > XXM > XYF ~ XYM

#AJPHeartCirc 2025
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How are #EndothelialDysfunction & #ArterialStiffness are coupled in #vascularAging ?👇

Biomechanical #Aging of Aortas of eNOS -/+ -/- 🐭
1.5~12-mo
Beyond Press+Flow

Wall geometry + matrix architecture
Biaxial Active + passive mechanics

#AJPHeartCirc 2025
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Guidelines for Assessing Ventricular Pressure-Volume Relationships in Rodents | AJP-Heart and Circulatory Physiology Podcast In this episode, Deputy Editor Dr. Zamaneh Kassiri (University of Alberta) interviews authors Dr. Oliver H. Wearing (University of British Columbia), Dr. Naomi C. Chesler (University of California Irv...

*NEW EPISODE* The #AJPHeartCirc Podcast 🎧

Listen as they discuss #physiology #guidelines for assessing #ventricular #PressureVolume relationships in rodents and how PV loops provide a complement to echocardiography and validate physiological measures. 🧪🎙❤️

ajpheart.podbean.com/e/guidelines...

#ExtracellularAcidification #SmoothMuscleCell

pH-sensing, acidification-activated GPR68➡️
Ca2+/PKCα➡️
Rap1A/B (How?🧐)➡️
SMC proliferation/Neointima formation

#ajpheartcirc 2024
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#EndothalialCell transcriptional regulator in👤#HeartFailure

26 WGCNA modules, module 17/21/22/23 Neg corr. with heart failure trait

3 transcription factors in VEGF-responsive module 23
CASZ1
ZNF532
NFE2L1/NRF1

#ajpheartcirc 2024
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#Piezo2 as Pulmonary Microvascular #EndothelialCell shear sensor

Piezo2, Not #Piezo1, downregulated in
1⃣EC + Hypoxia/TGFβ2
2⃣4 animal #PulmonaryHypertension
3⃣Human iPAH🫁EC

siRNA Piezo2->#EndMT

Daphne Merkus lab #AJPHeartCirc 2022
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#MatrixStiffness #Senescence

Passage-dependent senescent responses of cultured #CardiacFibroblast to Stiffness

Stiff hydrogel: 41 kPa
Soft: 6 kPa

Soft matrix⏫senescence (P2), ⏬TGFβ & ⏫NFκB-SASP (P5-6)

Timothy McKinsey lab #AJPHeartCirc 2023
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