#IscB: The "Pocket-Sized" Future of Plant #GenomeEditing 📖✨
Evolutionary ancestors are making a comeback! Scientists have optimized IscB—the hypercompact predecessor of #Cas9 —for high-efficiency work in Rice.
Read the full paper: link.springer.com/article/10.1...

Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature Cas9 structures explain topology sensing and off-target activation.

1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵

🔗 www.nature.com/articles/s41...

#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics

Multiplex #CRISPR library for #Arabidopsis

2 #sgRNAs/construct → 3.03 genes/vector → 84.2% editing (vs. 48.4%)

Tissue-specific #Cas9 (5 promoters). Barcode tracking: 1,014 plants, 4.16 plants/gene.

SWEET11-14 quad KO → novel phenotype

CRISPys + MOFF + GuideMap

📄 doi.org/10.1016/j.ce...

🚀 We're excited to announce that our new issue is now online!
5️⃣ Featuring: 2 articles honouring #WomenInScience, a state-of-the-art Review, a Viewpoint, an Editor's Choice with a Commentary, several original articles.
Discover ➡️https://buff.ly/XIqf1Na
#lipidation #Cas9 #ubiquitin #structuralbiology

🦠 100× boost in algae #CRISPR #knockout via dsNHO "decoys"
Co-delivery of 24bp non-homologous DNA sequesters KU proteins → forces MMEJ pathway → 0.29% → 44.5% efficiency in Chlamydomonas.
Works with #Cas9 & #Cas12a.
Read the full study:
onlinelibrary.wiley.com/doi/10.1111/...

HUH-tagged Cas9 as a platform for efficient ssODN-mediated knock-in via embryo and adult injection in insects - Communications Biology An HUH endonuclease–tagged Cas9 markedly enhances both knockout and knock-in efficiency in insects. By enabling efficient genome editing via adult and embryo injection, this platform expands precise g...

✂️🪲Injection-based insect genome editing: fusing a HUH endonuclease tag to #Cas9 boosts both #NHEJ ‑driven knockout and HDR‑mediated #knock‑in across multiple arthropods.
www.nature.com/articles/s42...

Turku Bioscience Genome Editing Core is organizing a workshop on generating knock-out and knock-in modification using CRISPR/Cas9 technology with in vitro ribonucleoprotein complex delivery.

Read more & apply here: bioscience.fi/events/crisp...

#geneediting #crispr #cas9

🧬 CRISPR technology is transforming genetics 🔬, enabling precise gene editing for research, diagnostics, and future therapeutic innovations 🚀.

#CRISPR #GeneEditing #Biotechnology #MolecularBiology #Cas9 #Genomics #LifeScienceStudents #ResearchInnovation #BiotechTrends #BioResire

The #FreeToRead #EditorsChoice article for the issue investigated the influence of IR25a on #electrophysiology and reproductive #behaviour in the #OrientalFruitFly, #Bactrocera dorsalis.

Why not give it a read?
doi.org/10.1111/imb.70005

#PestManagementStrategies #CRISPR #Cas9

Amplified Genome Editing by In Vivo Editor Production and Transfer to Non-Transduced Cells – Genome editing enzymes have vast therapeutic potential. However, achieving sufficient delivery in vivo remains a major challenge, because editing machinery is confined to the subset of transfectable cells in a tissue. Here, we tested the possibility that genome editing could be amplified in vivo by programming transfected cells to produce and transfer editing enzymes in lipid vesicles to neighboring cells. Our data show that this NANoparticle-Induced Transfer of Enzyme (NANITE) strategy tripled editing efficiency in cultured cells relative to non-spreading controls.   Furthermore, a single intravenous injection of the NANITE plasmid into mice induced ~3-fold higher levels of liver editing at the Ttr locus relative to non-spreading controls, with corresponding reductions in serum transthyretin levels. Amplifying therapeutic enzymes in situ offers a nonviral and non-infectious strategy to overcome low delivery efficiencies and reduce effective dose requirements.   Published in bioRxiv (January 13, 2026): https://doi.org/10.64898/2026.01.13.699115 

A novel technology developed in the laboratory of Nobel Prize laureate Jennifer Doudna promises to once again revolutionize in vivo genome editing and gene therapy approaches #genetherapy #geneediting #Cas9

Directed evolution of compact RNA-guided nucleases for homology-directed repair in mammalian cells RNA-guided nucleases enable DNA editing and offer promise for treating genetic diseases, particularly when used for precise sequence replacement. However, many of the most effective enzymes, such as S...

Sometimes #Cas9 is too dang big. But mini Cas proteins are bad at both NHEJ & HDR. PhD student Fedor Gorbenko used mammalian cell evolution for *HDR* to make REALLY super #Cas12f1 and #TnpB. As good as or better than Cas9! Base editors included! www.biorxiv.org/cgi/content/short/2025.10.27.684765v1

Checkout #SignalchemDx comprehensive portfolio of #CRISPR-associated #proteins inc highly active enzymes of exceptional specificity efficiency & versatility for the development of innovative & reliable CRISPR-based gene manipulation techniques 

🔗hhttps://tinyurl.com/582d6akz

#CAS9 #genomics

#ForestryRes
Unlocking larch genome with CRISPR/Cas9 🌱🔬 - DNA-free, precise, and efficient. No more genomic integration worries! 🚀 Ready for conifer revolution.
Details: www.maxapress.com/article/doi/10.48130/for...
#crispr #cas9 #genome #dna

Modified Cas9 Proteins Cut Prime-Editing Errors by Up to Six‑Fold

MIT engineers created a modified Cas9 variant that cuts prime‑editing errors from about one in seven edits to roughly one in 101 in standard workflows, and to one in 543 in high‑precision mode. getnews.me/modified-cas9-proteins-c... #geneediting #cas9

CRISPR study reveals surprising role of Cas9 as guardian of bacterial defense When scientists discovered how bacteria protect themselves against viral invaders, called phages, in the early 2000s, little did they know they'd stumbled upon a revolutionary tool researchers could…

#CRISPR study reveals surprising role of #Cas9 as guardian of #bacterial defense ...

| #bacteria | #virus | #phage | #spacers | #DNA | #RNA | By @umich.edu via @sciencex.bsky.social

🚀New from our lab @biorxivpreprint.bsky.social!
We reveal a cryptic metal binding pocket that dinamically forms in #CRISPR-Cas9 and regulates the metal-dependent activity of #Cas9.
#MarkovModels & #QuantumChemistry corroborated by biochem & #NMR experiments.
www.biorxiv.org/content/10.1...

Frontiers | CATS: a bioinformatic tool for automated Cas9 nucleases activity comparison in clinically relevant contexts IntroductionWith the growing number of Cas9 nucleases available to genetic engineers, selecting the most suitable one for a given application can be challeng...

Say hello to CATS, our new #bioinformatic tool that automates the comparison of #Cas9 nucleases and identification of #allele-specific #targets for mutations, making #CRISPR-Cas9 design faster and more precise!
GitHub: github.com/Physics4Medi...
Paper: www.frontiersin.org/journals/gen...

New research in #RESInsectMolecBio

The homeobox gene Distal-less is critical for the development of adult body appendages in #Bombyx mori
doi.org/10.1111/imb.13015

#InsectDevelopment #CRISPR #Cas9 #RNAseq
@sassanasgari.bsky.social @gulianusslab.bsky.social @wiley.com

3/ Too much #Cas9? You risk toxic effects and off-target cutting. Too little? Your editing efficiency plummets.

A novel rapid, sensitive, cost-effective, and non-radioactive method for Cas9 gRNA detection using a fluorophore-labeled DNA oligo.

Watch this video to learn more as Dr. Hu and his team explain the methodology.

Full-text here: doi.org/10.1016/j.ge...

#CRISPR #Cas9 #GeneEditing

Kevin Wasko (left) and Honglue Shi in front of their gel imager, confirming that the SpRY Cas9 variant struggles to effectively open DNA

“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author Honglue Shi of the @doudna-lab.bsky.social on his recent paper in Molecular Cell

Read here: innovativegenomics.org/uncovering-c...

Impressive spacer selection mechanism by Cas9-adaptation supercomplexes - all pathway captured by #cryoEM #Cas9 🧬🧩🧵

The function of the ring-shaped protein Csn2 is reminiscent of the sliding clamp in DNA replication. Repurposing of #Cas9 nuclease by the #CRISPR adaptation machinery for prespacer selection characterized here demonstrates #Cas9 plasticity.

Upon PAM recognition the DNA-scanning supercomplex adopts the 'docked' conformation. Its further rearrangement via displacement of #Cas9 REC2 domain yields the 'locked' supercomplex with straight DNA.

Here, we present #cryoEM structures of the type II-A prespacer selection supercomplex consisting of #Cas9 RNP, Cas1-Cas2 #integrase fragment and Csn2 ring in the DNA-scanning and two different PAM-bound configurations.

In type II-A #CRISPR-Cas systems acquisition of new spacers involves the effector nuclease #Cas9, which forms a ‘supercomplex’ with the Cas1-Cas2 #integrase and the ring-shaped Csn2 protein. The supercomplex structure and the role of Csn2 remain unknown.

The next speaker is selected from the submitted abstracts and is Deja Porenta who is working on assessing the Cas9-directed immune response in mice at the @utrechtuniversity.bsky.social #NVGCT2025 #LNPs #Cas9 #nanoparticles

Researchers find CRISPR is capable of even more than we thought Newly discovered weapons of bacterial self-defense take different approaches to achieving the same goal: preventing a virus from spreading through the bacterial population.

#CRISPR capable of even more than we thought ...

| #Bacteria | #Cas9 | #virus | #RNA | By @rockefelleruniv.bsky.social‬ via @eurekalert.bsky.social‬

In the last couple of decades, scientists revealed many details on the microbial #ImmuneSystem which protects them against their own #pathogens, like #phages, for example. One of the unexpected outcomes of these discoveries is the development of the #CRISPR - #Cas9 system used for #GeneEditing.

Noor Al-Sayyad

“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – @honglue.bsky.social of the @doudna-lab.bsky.social on his paper in Molecular Cell.

Read here: shorturl.at/RoKgG

Pictured: Co-first author Noor Al-Sayyad