New research in #GENETICS used loss-of-function assays to demonstrate the indispensable functions of unsaturated fatty acids in cell cycle progression, chromosome organization, and membrane architecture during #Celegans #germline maintenance. buff.ly/jSkjYlA
Prof. Yangqing Lu team from #Guangxi University established an induced infertile #chicken line for efficient #germline transmission of exogenous primordial germ #cells (#PGCs)
🐥🧬https://doi.org/10.1016/j.jia.2024.08.009
#animalscience
🩸 Questions of the day!
🧬 The first question explores #germline marrow failure syndromes, including #GATA2 mutations and inherited #MDS risk.
🔎 The second question looks at VEXAS syndrome in the context of marrow failure.
Swipe for answers and explanations!
#Hematology #BoneMarrowFailure #IBMFS
#quotes #books #booksky #excerpts #citations #crisprpeople #crispr #gene #edit #china #genome #germline #humangenome #ethics #consent #science #molbio #he
HZL-1 mRNA binding reverses argk-1-dependent modulation of TOR activity. Top left: Confocal micrograph images of daf-2; aak(0) animals with DAF-15::mNG::AID, i.e., the DAF-15 degron. Animals grown on control plates (without auxin) (Top) express mNG in the germ line (within the white lines). DAF-15 localizes to discrete puncta, presumably lysosomes, as has been previously shown (white arrows). mNG signal is absent in animals exposed to 100 µm IAA (Bottom). White asterisks denote non-specific signal from autofluorescence. Scale bar = 10 µm. Top right: Confocal images showing representative germ lines of dauer daf-2 and daf-2, aak(0) DAF-15 degron animals with and without auxin, following DAPI staining. Bottom: Model of AMPK-mediated regulation of HZL-1 and TOR. In the wild type, AMPK inhibits HZL-1, allowing for HZL-1-target mRNAs, such as argk-1, to promote germ cell quiescence. argk-1 negatively regulates TOR, and when argk-1 is bound by HZL-1, TOR activity is elevated. This drives the unscheduled germ cell divisions and subsequent hyperplasia observed in AMPK mutants during periods of energy challenge, resulting in post-dauer sterility.
#Celegans adapts to starvation & other stressors by transiting through the quiescent #dauer stage, but how? This study shows that TOR activity is inhibited by AMPK & a novel RNA-binding helicase in the #GermLine during dauer, preserving developmental quiescence @plosbiology.org 🧪 plos.io/48NEHvc
HZL-1 mRNA binding reverses argk-1-dependent modulation of TOR activity. Top left: Confocal micrograph images of daf-2; aak(0) animals with DAF-15::mNG::AID, i.e., the DAF-15 degron. Animals grown on control plates (without auxin) (Top) express mNG in the germ line (within the white lines). DAF-15 localizes to discrete puncta, presumably lysosomes, as has been previously shown (white arrows). mNG signal is absent in animals exposed to 100 µm IAA (Bottom). White asterisks denote non-specific signal from autofluorescence. Scale bar = 10 µm. Top right: Confocal images showing representative germ lines of dauer daf-2 and daf-2, aak(0) DAF-15 degron animals with and without auxin, following DAPI staining. Bottom: Model of AMPK-mediated regulation of HZL-1 and TOR. In the wild type, AMPK inhibits HZL-1, allowing for HZL-1-target mRNAs, such as argk-1, to promote germ cell quiescence. argk-1 negatively regulates TOR, and when argk-1 is bound by HZL-1, TOR activity is elevated. This drives the unscheduled germ cell divisions and subsequent hyperplasia observed in AMPK mutants during periods of energy challenge, resulting in post-dauer sterility.
#Celegans adapts to starvation & other stressors by transiting through the quiescent #dauer stage, but how? This study shows that TOR activity is inhibited by AMPK & a novel RNA-binding helicase in the #GermLine during dauer, preserving developmental quiescence @plosbiology.org 🧪 plos.io/48NEHvc
HZL-1 mRNA binding reverses argk-1-dependent modulation of TOR activity. Top left: Confocal micrograph images of daf-2; aak(0) animals with DAF-15::mNG::AID, i.e., the DAF-15 degron. Animals grown on control plates (without auxin) (Top) express mNG in the germ line (within the white lines). DAF-15 localizes to discrete puncta, presumably lysosomes, as has been previously shown (white arrows). mNG signal is absent in animals exposed to 100 µm IAA (Bottom). White asterisks denote non-specific signal from autofluorescence. Scale bar = 10 µm. Top right: Confocal images showing representative germ lines of dauer daf-2 and daf-2, aak(0) DAF-15 degron animals with and without auxin, following DAPI staining. Bottom: Model of AMPK-mediated regulation of HZL-1 and TOR. In the wild type, AMPK inhibits HZL-1, allowing for HZL-1-target mRNAs, such as argk-1, to promote germ cell quiescence. argk-1 negatively regulates TOR, and when argk-1 is bound by HZL-1, TOR activity is elevated. This drives the unscheduled germ cell divisions and subsequent hyperplasia observed in AMPK mutants during periods of energy challenge, resulting in post-dauer sterility.
#Celegans adapts to starvation & other stressors by transiting through the quiescent #dauer stage, but how? This study shows that TOR activity is inhibited by AMPK & a novel RNA-binding helicase in the #GermLine during dauer, preserving developmental quiescence @plosbiology.org 🧪 plos.io/48NEHvc
The need to move forward--responsibly--on treating devastating monogenic diseases. #bioethics #germline www.linkedin.com/posts/manhat...
RESEARCH PAPER: A cell type-specific surveillance complex represses cryptic promoters during differentiation in an adult stem cell lineage
By Matias et al. and Margaret Fuller
➡️ genesdev.cshlp.org/content/39/21-22/1318.ab...
#chromatin #transcription #Drosophila #stemcells #germline
What’s new in VDJbase 2025?
• 3000+ human + macaque samples
• IGH, IGK, IGL, IGHC, TRB loci
• Matched AIRR-seq and genomic data
• Population-stratified genotypes + haplotypes
• Repertoire-based zygosity calls
#AIRR #VDJbase #Germline
Online now! EGFR, JNK & ROS signaling mediate bidirectional feedback between cyst cells and encapsulated germ cells in Drosophila testis. #germline #polarity #endocytosis #cellcommunication ow.ly/cK0o50Xeakc
@isscr.org @gairdnerawards.bsky.social @sickkidsvs.bsky.social @cellpress.bsky.social
"The germline is the only cell type responsible for transmitting the genome from generation to generation," @whiteheadinstitute.bsky.social 's Dr. Yukiko Yamashita says.
⏳ Learn about how the Yamashita lab is teasing apart how the #germline remains immortal! https://bit.ly/42QARy3
In #celegans, #lysosomes signal through the #epigenome to boost #transgenerational longevity.
Lipid & AMPK signals, or reduced mTOR, reprogram histone H3.3, which is transported from the #intestine to the #germline, extending #lifespan across generations.
www.science.org/doi/10.1126/...
So proud we could take part in this amazing project!
Germline/meiosis crowd, you must check this out. A whole new dimension of non-cell autonomous control on gametogenesis.
Also, triple shoutout for @ethanewe.bsky.social . A great PI on the way
#meiosis #germline #ncRNA
DNA methylation at #retrotransposons protects the #germline by preventing NRF1-mediated #activation.
-> www.embopress.org/doi/full/10....
The August issue of @jmdiagn.bsky.social is now live online!
Full issue: https://www.jmdjournal.org/issue/S1525-1578(24)X0010-0
#germline #genomicprofiling #mutation #
Happy to share my recent clipboard article link.springer.com/article/10.1...
Thanks to @kavita-babu.bsky.social and @wormlockholmes.bsky.social
for giving me this wonderful opportunity! #germline #mechanics #cellmorphogenesis #Organ-leveldynamics
¿Qué piensas de la edición génica en humanos? Herramientas como CRISPR-Cas9 ofrecen múltiples posibilidades, pero también dilemas éticos, especialmente cuando se trata de alterar la línea germinal.
www.sciencedirect.com/science/arti...
#bianorbiotech #Germline #GeneCorrection #CRISPR #ethics
In #GENETICS, Spaulding and @dustinupdike.bsky.social tested how the full-length arginine-glycine repeats in #Celegans #germline organize into nucleolar subcompartments, thereby determining how proteins with these repeats influence nucleolar organization. 🪱
Read more: buff.ly/aRIjtRx
#DNA #methylation #4mC #epigenetics #germline #Marchantia #sperm #fertility #chromatin #reproduction #plantbiology #evolution
#quotes #books #booksky #excerpts #citations #CRISPRpeople #greely #HeJiankui #crispr #human #genome #ccr5 #hiv #mutation #geneediting #germline #ethics
March is #ColorectalCancer Awareness Month—a time to highlight the power of genomic intelligence in oncology. In our latest blog we explore how #germline (APC, MUTYH) & #somatic (BRAF, KRAS, NRAS) insights refine diagnosis & treatment. Read more: www.genomenon.com/blog/the-cri...
Genomics :: Governance, Ethics, Policy, Practice: A Monthly Digest January 2025 Number 11
ge2p2global-centergenomicmedicinegovernance.org/2025/02/20/2...
#genomics #genomicmedicine #geneediting #genetherapy #bioethics #governance #raredisease #germline
GE2P2 Global Foundation www.ge2p2.org
#quotes #books #excerpts #citations #he #crispr #gene #editing #embryo #mammal #human #geneediting #molecularengineering #unethical #germline
We are currently #hiring a faculty member in Regenerative and Aging Biology to address fundamental biological questions in the areas of #stemcell & #tissueregeneration, #germline development, ribonucleoprotein (RNP) assemblies, and #aging: 🧪🤝🧬
mdibl.org/careers/assi...
